来自埃塞俄比亚西北部德布雷塔博尔综合专科医院不同临床样本的 的抗菌药物耐药谱。
Antimicrobial Resistance Profile of from Different Clinical Samples in Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia.
机构信息
Department of Medical Microbiology, College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, Ethiopia.
Department of Medical Laboratory Sciences, College of Medicine and Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia.
出版信息
Ethiop J Health Sci. 2023 May;33(3):423-432. doi: 10.4314/ejhs.v33i3.5.
BACKGROUND
Pseudomonas aeruginosa is one of the leading causes of hospital-acquired infections and the most common antimicrobial-resistant pathogens. It is associated with a variety of infections. This study aimed to determine the prevalence of P. aeruginosa and its antimicrobial resistance profile from different clinical specimens at Debre Tabor Comprehensive Referral Hospital (DTCRH).
METHODS
A cross-sectional study was conducted from May to July 2022 at DTCRH. Socio-demographic and clinical data were collected using a structured questionnaire. Clinical samples (blood, wound swab, urine, and sputum) were collected from 348 study participants and processed following the standard bacteriological techniques. Antibiotic susceptibility testing was done by the Kirby-Bauer disc diffusion method. Data were entered and analyzed using SPSS version 25 statistical software. Descriptive statistics was used to present the findings of the study.
RESULTS
The prevalence of P.aeruginosa was 74(19.3%). The detection of the isolates was different based on the type of samples that ranged from 0% to 54.5% from sputum and wound swabs, respectively. P.aeruginosa showed resistance against gentamicin at 62.2%, ceftazidime 51.4%, cefepime 50%, amikacin 29.7%, imipenem 28.4% and ciprofloxacin 14.9%. The level of multi-drug resistance (MDR) was 45.9%, and the suspicious extreme-drug resistance (XDR) rate was 9.5%. Being inpatient and wound swab samples were factors associated with the detection of P.aeruginosa from clinical samples.
CONCLUSION
The antibiotic resistance profile of P. aeruginosa isolates in the present study area was found to be alarming. Actions to minimize the effect of antimicrobial resistance should be strengthened, and further large-scale study should be conducted to find out the main reasons behind antibiotic resistance of P.aeruginosa and other clinically relevant isolates.
背景
铜绿假单胞菌是医院获得性感染的主要原因之一，也是最常见的抗微生物药物耐药病原体。它与多种感染有关。本研究旨在确定来自德布雷塔博尔综合转诊医院（DTCRH）不同临床标本的铜绿假单胞菌的流行情况及其抗菌药物耐药谱。
方法
2022 年 5 月至 7 月在 DTCRH 进行了一项横断面研究。使用结构化问卷收集社会人口统计学和临床数据。从 348 名研究参与者中采集临床样本（血液、伤口拭子、尿液和痰液），并按照标准细菌学技术进行处理。采用 Kirby-Bauer 纸片扩散法进行抗生素敏感性试验。使用 SPSS 版本 25 统计软件输入和分析数据。使用描述性统计方法呈现研究结果。
结果
铜绿假单胞菌的检出率为 74（19.3%）。根据样本类型，分离株的检出率不同，痰液和伤口拭子的检出率分别为 0%至 54.5%。铜绿假单胞菌对庆大霉素的耐药率为 62.2%，头孢他啶为 51.4%，头孢吡肟为 50%，阿米卡星为 29.7%，亚胺培南为 28.4%，环丙沙星为 14.9%。多药耐药（MDR）率为 45.9%，可疑极端耐药（XDR）率为 9.5%。住院和伤口拭子样本是与从临床样本中检出铜绿假单胞菌相关的因素。
结论
本研究地区铜绿假单胞菌分离株的抗生素耐药谱令人担忧。应加强行动，以尽量减少抗微生物药物耐药的影响，并进一步开展大规模研究，以找出铜绿假单胞菌和其他临床相关分离株抗生素耐药的主要原因。
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