College of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Hangzhou Normal University, Hangzhou 311121, Zhejiang, China.
Anhui Topway Testing Services Co., Ltd., 18 Rixin Road, Xuancheng Economic and Technological Development Zone, Anhui 242000, China.
ACS Sens. 2022 Oct 28;7(10):3126-3134. doi: 10.1021/acssensors.2c01603. Epub 2022 Oct 7.
Tacrolimus (FK506) is widely used in the prevention of organ transplant rejection and the treatment of autoimmune diseases, but it is difficult to detect within the low and narrow concentration range in practical clinical fields. A magnetic plasmonic superstructure-targets-plasmonic superstructure-based sandwich-type SERS biosensor is presented here to ultrasensitively detect FK506 in the blood of organ transplant patients. The spiky FeO@SiO@Ag flower magnetic superstructure and hollow Ag@Au superstructure enhanced the SERS signals by providing rich sharp tips, cavities, and abundant hot spot gaps. And the magnetic feature makes it easy to concentrate and separate the biological target. Using the designed sandwich-type SERS biosensor, FK506 could be detected within a range of 0.5-20 ng/mL with a detection limit of 0.33 ng/mL. All results indicated that the sandwich-type SERS biosensor has good stability, sensitivity, and anti-interference properties. It is noteworthy that this allowed us to successfully analyze FK506 in the blood of transplant patients, which is in strong agreement with the clinical results. Consequently, the attractive sandwich-type SERS biosensor can be used for the detection of FK506 in real samples, which is promising for clinical diagnosis.
他克莫司（FK506）广泛用于器官移植排斥反应的预防和自身免疫性疾病的治疗，但在实际临床领域中，其在低浓度和窄浓度范围内难以检测。本文提出了一种基于磁等离子体超结构-靶-等离子体超结构的三明治型 SERS 生物传感器，用于超灵敏检测器官移植患者血液中的 FK506。多刺的 FeO@SiO@Ag 花状磁性超结构和空心 Ag@Au 超结构通过提供丰富的尖锐尖端、空腔和丰富的热点间隙增强了 SERS 信号。并且磁性特征使其易于浓缩和分离生物靶标。使用设计的三明治型 SERS 生物传感器，FK506 可以在 0.5-20 ng/mL 的范围内进行检测，检测限为 0.33 ng/mL。所有结果表明，三明治型 SERS 生物传感器具有良好的稳定性、灵敏度和抗干扰性。值得注意的是，这使得我们能够成功地分析移植患者血液中的 FK506，与临床结果非常吻合。因此，这种有吸引力的三明治型 SERS 生物传感器可用于实际样品中 FK506 的检测，有望用于临床诊断。
