Yang Haijun, Zhou Jiahuan, He Keyao, Li Junkuo, Zhao Fang, Dai Ningtao, Wu Shouxin, Li Wushuang, Zhao Jiangman, Zhang Yaowen, Zhou Fuyou
Anyang Tumor Hospital, The Affiliated Anyang Tumor Hospital of Henan University of Science and Technology, Anyang 455000, Henan, China.
Henan Key Medical Laboratory of Precise Prevention and Treatment of Esophageal Cancer, Anyang 455000, Henan, China.
J Cancer. 2023 Jul 31;14(12):2361-2372. doi: 10.7150/jca.84307. eCollection 2023.
This study aims to explore the role of in esophageal squamous cell carcinoma (ESCC) cells, determine the mRNA level of three isoforms in ESCC tissue, and evaluate the prognostic value of three isoforms. Colony-forming assay, Wound-healing assay and Transwell assay were used to evaluate the effect of on cell proliferation, migration and invasion. The mRNA expression of three isoforms was detected in paired tumor and normal tissues from 100 ESCC patients by real-time PCR. Kaplan-Meier survival curves and Cox proportional hazards model were used to evaluate the prognostic value of three isoforms. A nomogram was used to predict the probability of 2-year and 5-year overall survival (OS). In vitro, knockdown of could promote ESCC cell proliferation, migration and invasion abilities. Compared to the paired normal tissues, isoform 1 (, =0.0027) and isoform 2 (, =0.0006) were significantly decreased in tumor tissues. The low expression of mRNA was associated with advanced stage (=0.0176) and lymph node metastasis (LNM, =0.0219). ESCC patients with low mRNA levels had shorter survival time compared to those with high levels (=0.007). Multivariate COX analysis indicated that mRNA level was an independent prognostic indicator of OS of patients with ESCC (hazard ratio=0.5266, =0.03554). The concordance index of nomogram to predict OS was 0.693 based on LNM, , tumor stage and patients' age. These findings show that gene play a role in preventing proliferation, migration, and invasive activity of ESCC cells. mRNA level is a novel prognostic marker in ESCC, targeting may provide a potential therapeutic approach in ESCC.
本研究旨在探讨[具体物质]在食管鳞状细胞癌（ESCC）细胞中的作用，测定ESCC组织中三种[具体物质]亚型的mRNA水平，并评估三种[具体物质]亚型的预后价值。采用集落形成试验、伤口愈合试验和Transwell试验评估[具体物质]对细胞增殖、迁移和侵袭的影响。通过实时PCR检测100例ESCC患者配对的肿瘤组织和正常组织中三种[具体物质]亚型的mRNA表达。采用Kaplan-Meier生存曲线和Cox比例风险模型评估三种[具体物质]亚型的预后价值。使用列线图预测2年和5年总生存（OS）概率。在体外，敲低[具体物质]可促进ESCC细胞的增殖、迁移和侵袭能力。与配对的正常组织相比，肿瘤组织中[具体物质]亚型1（[具体指标1]，P = 0.0027）和[具体物质]亚型2（[具体指标2]，P = 0.0006）显著降低。[具体物质]mRNA低表达与晚期（P = 0.0176）和淋巴结转移（LNM，P = 0.0219）相关。与[具体物质]mRNA水平高的ESCC患者相比，[具体物质]mRNA水平低的患者生存时间更短（P = 0.007）。多变量COX分析表明，[具体物质]mRNA水平是ESCC患者OS的独立预后指标（风险比=0.5266，P = 0.03554）。基于LNM、[具体指标3]、肿瘤分期和患者年龄，预测OS的列线图一致性指数为0.693。这些发现表明，[具体物质]基因在预防ESCC细胞的增殖、迁移和侵袭活性中发挥作用。[具体物质]mRNA水平是ESCC中的一种新型预后标志物，靶向[具体物质]可能为ESCC提供一种潜在的治疗方法。
