Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, School of Ethnic Medicine, Yunnan Minzu University, Kunming, People's Republic of China.
Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People's Republic of China.
Int J Nanomedicine. 2023 Aug 8;18:4541-4554. doi: 10.2147/IJN.S416706. eCollection 2023.
Peptide-based vaccines have broad application prospects because of their safety, simple preparation, and effectiveness, especially in the development of personalized cancer vaccines, which have shown great advantages. However, the current peptide-based vaccines often require artificial synthesis and intricate delivery technology, which increases the cost and complexity of preparation.
Here, we developed a simple technique for combining a peptide and a delivery system using the natural secretion system of bacteria. Specifically, we biosynthesized an antigenic peptide in bacteria, which was then extracellularly released through the bacterial secretory vesicles, thus simultaneously achieving the biosynthesis and delivery of the peptide.
The system utilizes the natural properties of bacterial vesicles to promote antigen uptake and dendritic cell (DC) maturation. Therefore, tumor-specific CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) responses were induced in TC-1 tumor-bearing mice, thereby efficiently suppressing tumor growth.
This research promotes innovation and extends the application of peptide-based vaccine biosynthesis technology. Importantly, it provides a new method for personalized cancer immunotherapy that uses screened peptides as antigens in the future.
基于肽的疫苗因其安全性、简单的制备和有效性而具有广泛的应用前景，尤其是在个性化癌症疫苗的开发方面，具有很大的优势。然而，目前基于肽的疫苗通常需要人工合成和复杂的递药技术，这增加了制备的成本和复杂性。
在这里，我们开发了一种使用细菌天然分泌系统将肽与递药系统结合的简单技术。具体来说，我们在细菌中生物合成抗原性肽，然后通过细菌分泌小泡将其体外释放，从而同时实现肽的生物合成和递药。
该系统利用细菌小泡的天然特性促进抗原摄取和树突状细胞（DC）成熟。因此，在 TC-1 荷瘤小鼠中诱导了肿瘤特异性 CD4 Th1 和 CD8 细胞毒性 T 淋巴细胞（CTL）应答，从而有效地抑制了肿瘤生长。
本研究促进了基于肽的疫苗生物合成技术的创新和应用拓展。重要的是，它为未来使用筛选出的肽作为抗原的个性化癌症免疫治疗提供了一种新方法。
