Department of Gastric Surgery, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin, China.
Department of Gastrointestinal Surgery, Liaocheng People's Hospital, Liaocheng, Shandong, China.
PeerJ. 2023 Aug 9;11:e15559. doi: 10.7717/peerj.15559. eCollection 2023.
EDIL3, which contains epidermal growth factor-like repeats and discoidin I-like domains, is a secretory protein that plays an important role in embryonic development and various illnesses. However, the biological function of EDIL3 in gastric cancer (GC) is still unclear. The objective of this research was to explore the role and potential mechanism of EDIL3 in GC.
In this study, we used the GEPIA, HPA, MethSurv, SMART, STRING, GeneMANIA, LinkedOmics TIMER, TIMER2.0, TISIDB, and RNAactDrug databases to comprehensively analyze the roles of EDIL3 in GC. To validate the findings, EDIL3 expression was measured in our collected GC tissues. Meanwhile, several experiments were performed to test the function of EDIL3 in GC.
We found that EDIL3 was highly expressed in GC and associated with adverse clinical features. assays revealed that EDIL3 promoted the proliferation, migration, and invasion of GC cells. The functions of EDIL3 and co-expression genes were significantly associated with extracellular structure organization and matrix receptor interaction. EDIL3 expression was positively associated with numerous tumor-infiltrating immune cells and their biomarkers.
This study determined that EDIL3 may function as an oncogene and is associated with immune infiltration in GC. EDIL3 could be used as a potential therapeutic target for GC.
EDIL3 含有表皮生长因子样重复和盘状结构域 I 样结构域，是一种分泌蛋白，在胚胎发育和各种疾病中发挥重要作用。然而，EDIL3 在胃癌（GC）中的生物学功能尚不清楚。本研究旨在探讨 EDIL3 在 GC 中的作用及潜在机制。
本研究综合利用 GEPIA、HPA、MethSurv、SMART、STRING、GeneMANIA、LinkedOmics TIMER、TIMER2.0、TISIDB 和 RNAactDrug 数据库全面分析 EDIL3 在 GC 中的作用。为验证结果，我们检测了收集的 GC 组织中 EDIL3 的表达。同时进行了几项实验来检测 EDIL3 在 GC 中的功能。
我们发现 EDIL3 在 GC 中高表达，并与不良临床特征相关。实验表明 EDIL3 促进了 GC 细胞的增殖、迁移和侵袭。EDIL3 的功能和共表达基因与细胞外结构组织和基质受体相互作用显著相关。EDIL3 的表达与大量肿瘤浸润免疫细胞及其标志物呈正相关。
本研究确定 EDIL3 可能作为一种癌基因在 GC 中发挥作用，并与免疫浸润相关。EDIL3 可作为 GC 的潜在治疗靶点。
