Department of Biology, Suleyman Demirel University, Isparta, 32260, Turkey.
The Vocational School of Health Services, Namık Kemal University, Tekirdağ, 59030, Turkey.
Future Microbiol. 2022 Jul;17:861-871. doi: 10.2217/fmb-2021-0174. Epub 2022 Jun 6.
To investigate the hypothesis that zeolites interfere with quorum-sensing (QS) systems of and by adsorbing -acyl homoserine lactone (AHL) signal molecules. QS inhibition by zeolite 4A was investigated using an AHL-based bioreporter assay. The adsorption of the AHLs was evaluated by performing inductively coupled plasma-optical emission spectroscopy and confirmed by Monte Carlo and molecular dynamic simulations. Zeolite 4A reduced the violacein production in by over 90% and the biofilm formation, elastase and pyocyanin production in by 87, 68 and 98%, respectively. Zeolite 4A disrupts the QS systems of and by means of adsorbing 3-oxo-C6-AHL and 3-oxo-C12-AHL signaling molecules and can be developed as a novel QS jammer to combat -related infections.
为了验证沸石通过吸附酰基高丝氨酸内酯 (AHL) 信号分子来干扰 和 的群体感应 (QS) 系统这一假说，研究人员采用基于 AHL 的生物报告器测定法研究了沸石 4A 对 QS 的抑制作用。通过电感耦合等离子体-光学发射光谱法评价了 AHL 的吸附作用，并通过蒙特卡罗和分子动力学模拟进行了验证。沸石 4A 使 中紫色素的产生减少了 90%以上，使 中生物膜形成、弹性蛋白酶和绿脓菌素的产生分别减少了 87%、68%和 98%。沸石 4A 通过吸附 3-氧代-C6-AHL 和 3-氧代-C12-AHL 信号分子来破坏 和 的 QS 系统，有望开发为一种新型的 QS 抑制剂来对抗 相关感染。
