Miao Weihao, Xiao Xiangyu, Wang Yuean, Ge Lijiao, Yang Yanrong, Liu Ye, Liao Yuan, Guan Zhiyong, Chen Sumei, Fang Weimin, Chen Fadi, Zhao Shuang
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China.
Hortic Res. 2023 May 10;10(7):uhad101. doi: 10.1093/hr/uhad101. eCollection 2023 Jul.
Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry. However, the molecular mechanism underlying the response of chrysanthemum WRKY to infection remains largely unknown. In this study, we isolated from chrysanthemum 'Jinba' and identified it as a transcriptional repressor localized in the nucleus via subcellular localization and transcriptional activation assays. We found that negatively regulated resistance to and affected reactive oxygen species (ROS) and salicylic acid (SA) pathways using transgenic experiments and transcriptomic analysis. Moreover, bound to the W-box element on the promoter and inhibited its expression. Additionally, we observed that silencing in chrysanthemum reduced its resistance to via transgenic experiments. In conclusion, we revealed the mechanism underlying the CmWRKY6-1-CmWRKY15-like cascade response to infection in chrysanthemum and demonstrated that and regulates the immune system.
菊花枯萎病是一种土传病害，给菊花产业造成严重经济损失。然而，菊花WRKY对感染的响应分子机制仍 largely未知。在本研究中，我们从菊花‘津巴’中分离出[具体基因名称未给出]，并通过亚细胞定位和转录激活分析将其鉴定为定位于细胞核的转录抑制因子。我们发现[具体基因名称未给出]通过转基因实验和转录组分析负调控对[具体病原菌未给出]的抗性，并影响活性氧（ROS）和水杨酸（SA）途径。此外，[具体基因名称未给出]与[具体基因名称未给出]启动子上的W-box元件结合并抑制其表达。另外，我们通过转基因实验观察到菊花中[具体基因名称未给出]沉默降低了其对[具体病原菌未给出]的抗性。总之，我们揭示了菊花中CmWRKY6-1-CmWRKY15样级联反应对[具体病原菌未给出]感染的机制，并证明[具体基因名称未给出]和[具体基因名称未给出]调节免疫系统。
