CmWRKY6-1-CmWRKY15-like transcriptional cascade negatively regulates the resistance to infection in .

Chrysanthemum Fusarium wilt is a soil-borne disease that causes serious economic losses to the chrysanthemum industry. However, the molecular mechanism underlying the response of chrysanthemum WRKY to infection remains largely unknown. In this study, we isolated from chrysanthemum 'Jinba' and identified it as a transcriptional repressor localized in the nucleus via subcellular localization and transcriptional activation assays. We found that negatively regulated resistance to and affected reactive oxygen species (ROS) and salicylic acid (SA) pathways using transgenic experiments and transcriptomic analysis. Moreover, bound to the W-box element on the promoter and inhibited its expression. Additionally, we observed that silencing in chrysanthemum reduced its resistance to via transgenic experiments. In conclusion, we revealed the mechanism underlying the CmWRKY6-1-CmWRKY15-like cascade response to infection in chrysanthemum and demonstrated that and regulates the immune system.