State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of State Forestry and Grassland Administration On Biology of Ornamental Plants in East China, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
Zhongshan Biological Breeding Laboratory, No.50 Zhongling Street, Nanjing, 210014, Jiangsu, China.
BMC Genomics. 2023 Sep 19;24(1):553. doi: 10.1186/s12864-023-09671-0.
Black spot disease caused by the necrotrophic fungus Alternaria spp. is one of the most devastating diseases affecting Chrysanthemum morifolium. There is currently no effective way to prevent chrysanthemum black spot.
We revealed that pre-treatment of chrysanthemum leaves with the methy jasmonate (MeJA) significantly reduces their susceptibility to Alternaria alternata. To understand how MeJA treatment induces resistance, we monitored the dynamics of metabolites and the transcriptome in leaves after MeJA treatment following A. alternata infection. JA signaling affected the resistance of plants to pathogens through cell wall modification, Ca regulation, reactive oxygen species (ROS) regulation, mitogen-activated protein kinase cascade and hormonal signaling processes, and the accumulation of anti-fungal and anti-oxidant metabolites. Furthermore, the expression of genes associated with these functions was verified by reverse transcription quantitative PCR and transgenic assays.
Our findings indicate that MeJA pre-treatment could be a potential orchestrator of a broad-spectrum defense response that may help establish an ecologically friendly pest control strategy and offer a promising way of priming plants to induce defense responses against A. alternata.
由半知菌亚门链格孢属真菌引起的黑斑病是危害菊花的最严重病害之一。目前尚无有效方法预防菊花黑斑病。
我们发现，菊花叶片先用茉莉酸甲酯（MeJA）预处理可显著降低对Alternaria alternata 的易感性。为了了解 MeJA 处理如何诱导抗性，我们在 A. alternata 感染后监测了 MeJA 处理后叶片中代谢物和转录组的动态。茉莉酸信号通过细胞壁修饰、Ca 调节、活性氧（ROS）调节、丝裂原活化蛋白激酶级联和激素信号转导过程以及抗真菌和抗氧化代谢物的积累来影响植物对病原体的抗性，与这些功能相关的基因的表达通过反转录定量 PCR 和转基因实验得到了验证。
我们的研究结果表明，MeJA 预处理可能是一种广谱防御反应的潜在协调因子，有助于建立生态友好的害虫控制策略，并为诱导植物对 A. alternata 产生防御反应提供了一种有前景的方法。
