Hu Lingming, Ni Zhaojun, Zhao Kangqing, Li Xiangxue, Gao Xuejiao, Kang Yulin, Yu Zhoulong, Qin Ying, Zhao Jingwen, Peng Wenjuan, Lu Lin, Sun Hongqiang
Peking University Sixth Hospital, Peking University Institute of Mental Health, NHC Key Laboratory of Mental Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Peking University, Beijing, China.
Chinese Research Academy of Environmental Sciences, Beijing, China.
Front Microbiol. 2023 Jul 28;14:1203678. doi: 10.3389/fmicb.2023.1203678. eCollection 2023.
The relationship between oral and gut microbiota in alcohol dependence (AD) is not well understood, particularly the effects of oral microbiota on the intestinal microbiota. The current study aimed to explore the association between oral and gut microbiota in AD to clarify whether oral microbiota could ectopically colonize into the gut.
16S rRNA sequence libraries were used to compare oral and gut microbial profiles in persons with AD and healthy controls (HC). Source Tracker and NetShift were used to identify bacteria responsible for ectopic colonization and indicate the driver function of ectopic colonization bacteria.
The α-diversity of oral microbiota and intestinal microbiota was significantly decreased in persons with AD (all < 0.05). Principal coordinate analysis indicated greater similarity between oral and gut microbiota in persons with AD than that in HC, and oral-gut overlaps in microbiota were found for 9 genera in persons with AD relative to only 3 genera in HC. The contribution ratio of oral microbiota to intestinal microbiota composition in AD is 5.26% based on Source Tracker，and the AD with ectopic colonization showed the daily maximum standard drinks, red blood cell counts, hemoglobin content, and PACS scores decreasing (all < 0.05).
Results highlight the connection between oral-gut microbiota in AD and suggest novel potential mechanistic possibilities.
酒精依赖（AD）中口腔微生物群与肠道微生物群之间的关系尚未完全明确，尤其是口腔微生物群对肠道微生物群的影响。本研究旨在探讨酒精依赖患者口腔微生物群与肠道微生物群之间的关联，以阐明口腔微生物群是否会异位定植于肠道。
使用16S rRNA序列文库比较酒精依赖患者和健康对照（HC）的口腔和肠道微生物谱。使用Source Tracker和NetShift来识别负责异位定植的细菌，并指出异位定植细菌的驱动功能。
酒精依赖患者的口腔微生物群和肠道微生物群的α多样性显著降低（均<0.05）。主坐标分析表明，酒精依赖患者的口腔和肠道微生物群之间的相似性高于健康对照，酒精依赖患者中有9个属的微生物存在口腔-肠道重叠，而健康对照中只有3个属。基于Source Tracker，酒精依赖患者中口腔微生物群对肠道微生物群组成的贡献率为5.26%，发生异位定植的酒精依赖患者的每日最大标准饮酒量、红细胞计数、血红蛋白含量和PACS评分均降低（均<0.05）。
结果突出了酒精依赖中口腔-肠道微生物群之间的联系，并提出了新的潜在机制可能性。
