Liao Ying, Tong Xia-Ting, Jia Yi-Jing, Liu Qiao-Yun, Wu Yan-Xia, Xue Wen-Qiong, He Yong-Qiao, Wang Tong-Min, Zheng Xiao-Hui, Zheng Mei-Qi, Jia Wei-Hua
State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
School of Public Health, Sun Yat-sen University, Guangzhou 510062, China.
Int J Environ Res Public Health. 2022 May 8;19(9):5729. doi: 10.3390/ijerph19095729.
The dysbiosis of oral microbiota is linked to numerous diseases and is associated with personal lifestyles, such as alcohol drinking. However, there is inadequate data to study the effect of alcohol drinking on oral microbiota from the Chinese population. Here, we profiled the oral microbiota of 150 healthy subjects in the Chinese population by 16S rRNA gene sequencing. The results showed that drinkers had significantly higher alpha diversity than non-drinkers. A significant difference in overall microbiota composition was observed between non-drinkers and drinkers. Additionally, using DESeq analysis, we found genus and , and species and were significantly enriched in drinkers; meanwhile, the genus , and , and species were significantly depleted in drinkers. PICRUSt analysis showed that significantly different genera were mainly related to metabolism pathways. The oxygen-independent pathways, including galactose, fructose and mannose metabolism pathways, were enriched in drinkers and positively associated with genera enriched in drinkers; while the pyruvate metabolism pathway, an aerobic metabolism pathway, was decreased in drinkers and negatively associated with genera enriched in drinkers. Our results suggested that alcohol drinking may affect health by altering oral microbial composition and potentially affecting microbial functional pathways. These findings may have implications for better understanding the potential role those oral bacteria play in alcohol-related diseases.
口腔微生物群的失调与多种疾病相关，并且与个人生活方式有关，比如饮酒。然而，关于饮酒对中国人群口腔微生物群影响的研究数据并不充足。在此，我们通过16S rRNA基因测序对150名中国健康受试者的口腔微生物群进行了分析。结果显示，饮酒者的α多样性显著高于不饮酒者。在不饮酒者和饮酒者之间观察到整体微生物群组成存在显著差异。此外，使用DESeq分析，我们发现属 和 ，以及种 和 在饮酒者中显著富集；同时，属 、 和 ，以及种 在饮酒者中显著减少。PICRUSt分析表明，显著不同的属主要与代谢途径相关。包括半乳糖、果糖和甘露糖代谢途径在内的无氧代谢途径在饮酒者中富集，并且与饮酒者中富集的属呈正相关；而作为有氧代谢途径的丙酮酸代谢途径在饮酒者中减少，并且与饮酒者中富集的属呈负相关。我们的结果表明，饮酒可能通过改变口腔微生物组成并潜在影响微生物功能途径来影响健康。这些发现可能有助于更好地理解那些口腔细菌在酒精相关疾病中所起的潜在作用。
