State Key Laboratory of Chemical Oncogenomics, Guangdong Provincial Key Laboratory of Chemical Biology, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, P. R. China.
Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory, Shenzhen 518132, P. R. China.
Anal Chem. 2023 Aug 22;95(33):12216-12222. doi: 10.1021/acs.analchem.3c00565. Epub 2023 Aug 14.
In terms of cancer diagnoses and cancer-related deaths worldwide, colorectal cancer (CRC) is now the third most common malignancy. The drawbacks of current screening methods are their exorbitant costs, difficult procedures, and lengthy implementation timelines. The benefits of fecal screening for CRC are ease of operation, noninvasiveness, cost-effectiveness, and superior sensitivity. As a result of its enrichment in the malignant tissues and feces of CRC patients, () has emerged as a crucial biomarker for the incipient detection, identification, and prognostic prediction of CRC. Here, for the first time, the whole-bacterium SELEX method was used to screen the highly specific and affinity aptamers against by 13 cycles of selection. The Apt-S-5 linear correlation equation is = 0.7363 ( = 0.9864) with a limit of detection (LOD) of 851 CFU/mL. The results of the experiment using fecal samples revealed a substantial disparity between the microorganisms in the CRC patients' feces and those in the feces of healthy individuals and were consistent with those of qPCR. The aptamers may therefore offer a crucial approach to identifying and hold tremendous promise for CRC diagnosis and prognostic prediction.
在全球范围内，癌症的诊断和癌症相关死亡病例中，结直肠癌（CRC）现在是第三大常见恶性肿瘤。目前筛查方法的缺点是成本过高、程序复杂和实施时间周期长。CRC 粪便筛查的优点是操作简便、无创、具有成本效益，并且灵敏度较高。由于在 CRC 患者的恶性组织和粪便中富集，（）已成为 CRC 早期检测、识别和预后预测的重要生物标志物。在这里，首次通过 13 轮筛选，使用全菌 SELEX 方法筛选出针对的高特异性和高亲和力适体。Apt-S-5 线性相关方程为 = 0.7363（= 0.9864），检测限（LOD）为 851 CFU/mL。使用粪便样本进行的实验结果表明，CRC 患者粪便中的微生物与健康个体粪便中的微生物之间存在显著差异，并且与 qPCR 的结果一致。因此，适体可能为鉴定提供了一种重要方法，并为 CRC 诊断和预后预测带来了巨大的希望。
