Guangdong Engineering Research Center of Chinese Medicine & Disease Susceptibility, Jinan University, Guangzhou, China.
Methods Mol Biol. 2023;2712:81-90. doi: 10.1007/978-1-0716-3433-2_8.
Ferroptosis is a regulated form of cell death characterized by the accumulation of oxidized phospholipids, particularly oxidized phosphatidylethanolamines (PE), which serve as important biomarkers in the progression of various diseases. To facilitate the comprehensive investigation of ferroptosis in biological systems, we present a robust and versatile untargeted redox phospholipidomics method employing normal-phase liquid chromatography-mass spectrometry (LC-MS). This high-throughput technique enables the identification and quantification of dozens of oxidized phospholipid species in a single run, providing valuable insights into the molecular mechanisms underlying ferroptosis. It has been successfully applied to diverse biological samples, including human patients, animals, and cell cultures, and offers a powerful tool for investigating the roles of oxidized phospholipids in the development and progression of various diseases.
铁死亡是一种受调控的细胞死亡形式，其特征是氧化磷脂的积累，特别是氧化磷脂酰乙醇胺（PE），它们作为各种疾病进展的重要生物标志物。为了促进生物系统中铁死亡的全面研究，我们提出了一种使用正相液相色谱-质谱联用（LC-MS）的强大而通用的非靶向氧化磷脂组学方法。这种高通量技术可以在单次运行中鉴定和定量数十种氧化磷脂种类，为铁死亡的分子机制提供有价值的见解。它已成功应用于多种生物样本，包括人类患者、动物和细胞培养物，为研究氧化磷脂在各种疾病的发生和发展中的作用提供了有力工具。
