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,新种,来自空调冷凝水排水管道。

, sp. nov., from an air conditioner condensate drain line.

作者信息

Onouye T Chiyoko, Busse Hans-Jürgen, Prescott Rebecca D, Darris Maxwell K, Donachie Stuart P

机构信息

School of Life Sciences, University of Hawai'i at Mānoa, 1800 East-West Road, Honolulu, Hawai'i 96822, USA.

Institut für Mikrobiologie, Veterinärmedizinische Universität Wien, Veterinärplatz 1, A-1210 Wien, Austria.

出版信息

Int J Syst Evol Microbiol. 2023 Aug;73(8). doi: 10.1099/ijsem.0.006008.

Abstract

A Gram-negative, rod-shaped and filamentous bacterium designated MD30B was isolated from a biofilm hanging in water flowing from an air conditioner condensate drain line in Honolulu, Hawai'i. Based on 1517 nucleotides of the strain's 16S rRNA gene, its nearest neighbours are T16R-86 (96.7 %), S-52 (96.6 %), ZY74 (96.6 %), JS13-10 (96.6 %) and Gsoil 052 (96.5 %). MD30B cells are non-motile, strictly aerobic, and catalase and oxidase positive. Growth occurs between 10 and 45 °C. Major fatty acids in whole cells of MD30B are 13-methyl tetradecanoic acid (34.1 %), -11-hexadecenoic acid (30.3 %), and 3-hydroxy, 15-methyl hexadecanoic acid (13.3 %). The quinone system contains predominantly menaquinone MK-7. The polar lipid profile contains the major lipids phosphatidylethanolamine, one unidentified lipid lacking a functional group, and two unidentified aminolipids. -Homospermidine is the major polyamine. The G+C content of the genome is 47.58 mol%. Based on phenotypic and genotypic differences between MD30B and extant species in the , we propose that MD30B represents a new species, for which the name sp. nov. is proposed to accommodate strain MD30B as the type strain (DSM 112477=NCTC 14606).

摘要

从夏威夷檀香山空调冷凝水排水管道流出的水中悬挂的生物膜中分离出一株革兰氏阴性、杆状且呈丝状的细菌,命名为MD30B。基于该菌株16S rRNA基因的1517个核苷酸,其最相近的菌株为T16R - 86(相似度96.7%)、S - 52(96.6%)、ZY74(96.6%)、JS13 - 10(96.6%)和Gsoil 052(96.5%)。MD30B细胞无运动性,严格需氧,过氧化氢酶和氧化酶呈阳性。在10至45°C之间生长。MD30B全细胞中的主要脂肪酸为13 - 甲基十四烷酸(34.1%)、-11 - 十六碳烯酸(30.3%)和3 - 羟基、15 - 甲基十六烷酸(13.3%)。醌系统主要含有甲基萘醌MK - 7。极性脂质谱包含主要脂质磷脂酰乙醇胺、一种未鉴定的无功能基团脂质和两种未鉴定的氨基脂质。-高亚精胺是主要的多胺。基因组的G + C含量为47.58 mol%。基于MD30B与该属现有物种之间的表型和基因型差异,我们提出MD30B代表一个新物种,为此提议将sp. nov.作为该物种的名称,以容纳菌株MD30B作为模式菌株(DSM 112477 = NCTC 14606)。

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