Kim Soo-Jin, Cho Hayoung, Ahn Jae-Hyung, Weon Hang-Yeon, Joa Jae-Ho, Hong Seung-Beom, Seok Soon-Ja, Kim Jeong-Seon, Kwon Soon-Wo
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeju 63240, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3435-3439. doi: 10.1099/ijsem.0.002134. Epub 2017 Sep 6.
An aerobic, Gram-stain-negative, non-spore-forming, non-flagellated, rod-shaped or filamentous bacterial strain, T16R-86T, was isolated from rhizosphere of a tomato plant collected from a farm on Buyeo-gun, Chungcheongnam-do, South Korea. It grew at the temperature range 10-37 °C (optimum, 28 °C) and pH range 6.0-9.0 (optimum, pH 7.0), and tolerated up to 2 % (w/v) NaCl. According to 16S rRNA gene sequence analysis, strain T16R-86T shared the highest similarity with Chitinophaga barathri YLT18T (96.8 %) and C. pinensis DSM 2588T (96.7 %), forming a subcluster with C. barathri YLT18T, C. cymbidii R156-2T and C. niabensis JS13-10T in the phylogenetic tree. The major fatty acids were iso-C15 : 0, C16 : 1ω5c and iso-C17 : 0 3-OH. The predominant respiratory quinone was menaquinone MK-7. Polar lipids were phosphatidylethanolamine, five unknown aminolipids, an unknown aminophospholipid, one unknown phospholipid and two unknown lipids. The DNA G+C content was 53.6 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed that strain T16R-86T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga rhizosphaerae sp. nov. is proposed. The type strain is T16R-86T (=KACC 18790T=JCM 31600T).
从韩国忠清南道扶余郡一个农场采集的番茄植株根际分离出一株需氧、革兰氏染色阴性、不形成芽孢、无鞭毛、杆状或丝状的细菌菌株T16R - 86T。它在10 - 37 °C（最适温度28 °C）的温度范围内生长，在pH值6.0 - 9.0（最适pH值7.0）的环境中生长，并且能耐受高达2 %（w/v）的NaCl。根据16S rRNA基因序列分析，菌株T16R - 86T与巴氏噬几丁质菌YLT18T（96.8 %）和松噬几丁质菌DSM 2588T（96.7 %）具有最高的相似性，在系统发育树中与巴氏噬几丁质菌YLT18T、大花蕙兰噬几丁质菌R156 - 2T和尼亚宾噬几丁质菌JS13 - 10T形成一个亚群。主要脂肪酸为异C15 : 0、C16 : 1ω5c和异C17 : 0 3 - OH。主要呼吸醌为甲基萘醌MK - 7。极性脂质为磷脂酰乙醇胺、五种未知氨基脂质、一种未知氨基磷脂、一种未知磷脂和两种未知脂质。DNA G + C含量为53.6 mol%。表型、化学分类和系统发育数据表明，菌株T16R - 86T代表噬几丁质菌属的一个新物种，为此提出新名称根际噬几丁质菌（Chitinophaga rhizosphaerae sp. nov.）。模式菌株为T16R - 86T（=KACC 18790T=JCM 31600T）。
