Khanh Nguyen Van, Dutta Swarnalee, Kim Chang-Su, Lee Yong Hoon
Division of Biotechnology, Jeonbuk National University, Iksan-si, Republic of Korea.
Jinan Medicinal Resource Research Institute, Jeollabukdo Agricultural Research and Extension Services, Jinan-gun, Republic of Korea.
Front Microbiol. 2024 Apr 24;15:1389907. doi: 10.3389/fmicb.2024.1389907. eCollection 2024.
Symbiotic microbes are essential for developing and growing , an achlorophyllous orchid of high medicinal value. Recently, the cultivation of in greenhouses has been adopted in Korea to produce mature tubers in a short time. However, no studies have been conducted on the microbial community structure of cultivated in greenhouse environments. Therefore, we analyzed the temporal features of bacterial and fungal communities in the rhizosphere of at the juvenile [JT; 2 months after sowing (MAS)], young (YT; 6 MAS), and mature (MT; 11 MAS) tuber stages using culture-dependent and high-throughput sequencing technology. The richness and diversity of the bacterial and fungal communities decreased with tuber growth of The symbiotic fungi sp. and sp. as well as tuber extract inhibited the growth of various soil-inhabiting fungal and bacterial strains, indicating that and its symbiotic fungi play important roles in the selection of rhizosphere microbes. was the most abundant fungal species in the rhizosphere. We also identified the microorganisms potentially beneficial for development during greenhouse cultivation. Tubers and symbiotic fungi actively exert selective pressure on rhizosphere microbes, influencing the diversity and abundance of bacterial and fungal communities as grows. This study is a first report on the temporal microbial community structure of cultivated in greenhouse. The results on the associated microbiome of will help understand their beneficial interactions with and contribute to improvement in cultivation.
共生微生物对于高药用价值的非绿色植物兰花的发育和生长至关重要。最近，韩国采用了在温室中种植该兰花的方法，以便在短时间内生产成熟块茎。然而，尚未有关于温室环境中种植的该兰花微生物群落结构的研究。因此，我们使用依赖培养和高通量测序技术，分析了该兰花在幼年[JT；播种后2个月（MAS）]、幼年（YT；6 MAS）和成熟（MT；11 MAS）块茎阶段根际细菌和真菌群落的时间特征。随着该兰花块茎的生长，细菌和真菌群落的丰富度和多样性降低。共生真菌XX菌属和XX菌属以及块茎提取物抑制了各种土壤栖息真菌和细菌菌株的生长，表明该兰花及其共生真菌在根际微生物的选择中发挥着重要作用。XX菌是根际中最丰富的真菌物种。我们还确定了温室种植期间对该兰花发育可能有益的微生物。随着该兰花的生长，块茎和共生真菌对根际微生物积极施加选择压力，影响细菌和真菌群落的多样性和丰度。本研究是关于温室种植的该兰花时间微生物群落结构的首次报告。该兰花相关微生物组的结果将有助于理解它们与该兰花的有益相互作用，并有助于改进种植方法。
