Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi Arabia.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi Arabia.
J Infect Public Health. 2023 Oct;16(10):1613-1618. doi: 10.1016/j.jiph.2023.07.021. Epub 2023 Aug 2.
Hepatitis B Virus (HBV) is one of the leading causes of infectious disease in the global population, and its prevalence has been increasing globally. Human HBV infection is complex, involving both innate and adaptive immune systems. Cytokines play a role in both physiologic and pathologic processes in the liver. This study was designed to screen serum levels using an enzyme linked immunosorbent assay (ELISA) and genetic variants in the TNF-α and IL6 genes using polymerase chain reactions (PCR). The aim of this study was to screen the serum levels and genotype levels with TNF-α (C-308 T/G-308A) and IL-6 (G-174 C) genes in HBV patients and control subjects.
In this study, we have selected 50 HBV patients and 40 control subjects from Saudi Population. Patient serum samples was used for measuring the serum levels and PCR analysis using RFLP analysis. Prior to this, HBV patients were confirmed with PCR analysis followed by Sanger sequencing analysis.
The current study results confirmed positive association in serum levels (p < 0.05) and negative association with both genotype and allele frequencies in TNF-α (C-308 T) and IL-6 (G-174 C) genes among HBV patients and controls (p > 0.05). Positive associations between blood levels of TNF-α and IL-6 were confirmed, while negative associations were found between PCR investigations involving the TNF-α (G-308A) and IL-6 (G-174 C) genes with the HBV prevalence in the Saudi population.
This study confirmed serum levels are strongly associated with HBV patients in the Saudi population. However, PCR studies showed the negative association with the couple of variants selected for this study.
乙型肝炎病毒（HBV）是导致全球人群传染病的主要原因之一，其全球流行率一直在上升。人类 HBV 感染复杂，涉及固有和适应性免疫系统。细胞因子在肝脏的生理和病理过程中都发挥作用。本研究旨在通过酶联免疫吸附试验（ELISA）筛选血清水平，并通过聚合酶链反应（PCR）筛选 TNF-α 和 IL6 基因的遗传变异。本研究的目的是筛选 HBV 患者和对照组 TNF-α（C-308T/G-308A）和 IL-6（G-174C）基因的血清水平和基因型水平。
本研究从沙特人群中选择了 50 名 HBV 患者和 40 名对照者。使用患者血清样本测量血清水平，并使用 RFLP 分析进行 PCR 分析。在此之前，通过 PCR 分析和 Sanger 测序分析确认 HBV 患者。
本研究结果证实，HBV 患者的血清水平呈正相关（p<0.05），TNF-α（C-308T）和 IL-6（G-174C）基因的基因型和等位基因频率均呈负相关（p>0.05）。TNF-α 和 IL-6 血液水平之间存在正相关，而 TNF-α（G-308A）和 IL-6（G-174C）基因的 PCR 研究与 HBV 在沙特人群中的流行率呈负相关。
本研究证实了血清水平与沙特人群中的 HBV 患者密切相关。然而，PCR 研究显示，与本研究选择的几个变体呈负相关。
