扎伊德拉伸氏肝癌腹水细胞膜主要糖蛋白的O-糖苷寡糖链结构

The structure of the O-glycosidic oligosaccharide chains of the major Zajdela hepatoma ascites-cell-membrane glycoprotein.

作者信息

Nato F, Goulut C, Bourrillon R, Van Halbeek H, Vliegenthart J F

出版信息

Eur J Biochem. 1986 Sep 1;159(2):303-8. doi: 10.1111/j.1432-1033.1986.tb09868.x.

DOI:10.1111/j.1432-1033.1986.tb09868.x

Abstract

Glycoprotein MII2, the major cell surface glycoprotein (molecular mass 110 kDa) of Zajdela hepatoma ascites cells, contains about 25 O-glycosidic oligosaccharide chains per molecule. They were released as oligosaccharide-alditols by alkaline borohydride treatment of MII2, and purified by gel filtration on Bio-Gel P-6 followed by high-voltage paper electrophoresis. Four oligosaccharide-alditol fractions (A-D) were obtained in relative yields of 8:6:3:3. The structure of the components of fractions A-C was determined by 500-MHz 1H-NMR spectroscopy in combination with sugar composition analysis, to be as follows. (A) NeuAc alpha(2----3)Gal beta(1----3)[NeuAc alpha(2----3)Gal beta(1----4)GlcNAc beta(1----6)]GalNAc-ol; (B1) NeuAc alpha(2----3)Gal beta(1----3)[Gal beta(1----4)GlcNAc beta(1----6)]GalNAc-ol; (B2) Gal beta(1----3)[NeuAc alpha(2----3)Gal beta(1----4)GlcNAc beta(1----6)]GalNAc-ol; (C) NeuAc alpha(2----3)Gal beta(1----3)GalNAc-ol. On the basis of sugar composition and characteristics on Bio-Gel P-6 filtration, paper electrophoresis and thin-layer chromatography, the structure of the carbohydrate component of fraction D is proposed to be as follows. (D) NeuAc alpha(2----3)Gal beta(1----3)[NeuAc alpha(2----6)]GalNAc-ol

摘要

糖蛋白MII2是扎伊德氏肝癌腹水细胞的主要细胞表面糖蛋白（分子量110 kDa），每个分子含有约25条O-糖苷寡糖链。通过用碱性硼氢化物处理MII2将它们以寡糖糖醇的形式释放出来，并通过在Bio-Gel P-6上进行凝胶过滤，随后进行高压纸电泳来纯化。获得了四个寡糖糖醇级分（A - D），相对产率为8:6:3:3。通过500兆赫兹的1H-NMR光谱结合糖组成分析确定了级分A - C的成分结构，如下所示。（A）NeuAcα(2→3)Galβ(1→3)[NeuAcα(2→3)Galβ(1→4)GlcNAcβ(1→6)]GalNAc-ol；（B1）NeuAcα(2→3)Galβ(1→3)[Galβ(1→4)GlcNAcβ(1→6)]GalNAc-ol；（B2）Galβ(1→3)[NeuAcα(2→3)Galβ(1→4)GlcNAcβ(1→6)]GalNAc-ol；（C）NeuAcα(2→3)Galβ(1→3)GalNAc-ol。基于糖组成以及在Bio-Gel P-6过滤、纸电泳和薄层色谱上的特性，提出级分D的碳水化合物成分结构如下。（D）NeuAcα(2→3)Galβ(1→3)[NeuAcα(2→6)]GalNAc-ol