Center for Bio-Healthcare Materials, Bio-Convergence Materials R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, 28160, Republic of Korea.
Department of Chemical Engineering, Hanyang University, Seoul, 04763, Republic of Korea.
Int J Nanomedicine. 2023 Aug 9;18:4555-4565. doi: 10.2147/IJN.S401808. eCollection 2023.
Deinoxanthin (DX), a carotenoid, has excellent antioxidant and anti-inflammatory properties. However, owing to its lipophilicity, it is unfavorably dispersed in water and has low stability, limiting its application in cosmetics, food, and pharmaceuticals. Therefore, it is necessary to study nanoparticles to increase the loading capacity and stability of DX.
In this study, DX-loaded nanocapsules (DX@NCs) were prepared by nanoprecipitation by loading DX into nanocapsules. The size, polydispersity index, surface charge, and morphology of DX@NCs were confirmed through dynamic light scattering and transmission electron microscopy. The loading content and loading efficiency of DX in DX@NCs were analyzed using high-performance liquid chromatography. The antioxidant activity of DX@NCs was evaluated by DPPH assay and in vitro ROS. The biocompatibility of DX@NCs was evaluated using an in vitro MTT assay. In vitro NO analysis was performed to determine the effective anti-inflammatory efficacy of DX@NCs.
DX@NCs exhibited increased stability and antioxidant efficacy owing to the improved water solubility of DX. The in situ and in vitro antioxidant activity of DX@NCs was higher than that of unloaded DX. In addition, it showed a strong anti-inflammatory effect by regulating the NO level in an in vitro cell model.
This study presents a nanocarrier to improve the water-soluble dispersion and stability of DX. These results demonstrate that DX@NC is a carrier with excellent stability and has a high potential for use in cosmetic and pharmaceutical applications owing to its antioxidant and anti-inflammatory effects.
去甲叶黄素（DX）是一种类胡萝卜素，具有出色的抗氧化和抗炎特性。然而，由于其亲脂性，它在水中不易分散，且稳定性低，限制了其在化妆品、食品和制药领域的应用。因此，有必要研究纳米粒子以提高 DX 的负载能力和稳定性。
本研究通过将 DX 载入纳米胶囊来制备负载 DX 的纳米胶囊（DX@NCs），通过动态光散射和透射电子显微镜确认 DX@NCs 的粒径、多分散指数、表面电荷和形态。使用高效液相色谱分析 DX@NCs 中 DX 的载药量和载药效率。通过 DPPH 测定法和体外 ROS 评估 DX@NCs 的抗氧化活性。通过体外 MTT 测定法评估 DX@NCs 的生物相容性。通过体外 NO 分析测定 DX@NCs 的有效抗炎功效。
由于 DX 的水溶性得到改善，DX@NCs 表现出增强的稳定性和抗氧化功效。DX@NCs 的原位和体外抗氧化活性高于未负载的 DX。此外，它通过调节体外细胞模型中的 NO 水平表现出强烈的抗炎作用。
本研究提出了一种纳米载体来提高 DX 的水溶性分散和稳定性。这些结果表明，由于 DX@NC 具有出色的稳定性和抗氧化、抗炎作用，它是一种具有高潜力的用于化妆品和制药应用的载体。
