Wolfe J K, Chylack L T
Exp Eye Res. 1986 Aug;43(2):243-9. doi: 10.1016/s0014-4835(86)80092-6.
Metabolism in human senile cataracts has been studied using uniformly labeled [14C]glucose. Intracapsularly extracted lenses were cultured in TC-199 media with a glucose concentration of 5.5 mM. Results show that lactate production accounts for 97% of the glucose metabolized. Under these standard incubation conditions there is negligible accumulation of alpha-glycerol phosphate, glucose-6-phosphate, and sorbitol. The rate of lactate production was found to be relatively uniform over a range of cataract severities which were determined from the CCRG classification. The effects of several perturbants in the medium were measured. An ATP concentration of 3 mM was found to inhibit lactate production. Labeled glucose-6-phosphate in the medium was found to produce lactate at a rate approximately one half that of glucose. Elevated glucose concentration resulted in a slight decrease in lactate production and, in some lenses, production of a small amount of sorbitol. Overall, the glycolytic pathway appears to be functioning normally and without regard for cortical and nuclear opacification.
利用均匀标记的[14C]葡萄糖对人类老年性白内障的代谢进行了研究。将囊内摘除的晶状体在葡萄糖浓度为5.5 mM的TC-199培养基中培养。结果显示，乳酸生成占代谢葡萄糖的97%。在这些标准培养条件下，α-磷酸甘油、6-磷酸葡萄糖和山梨醇的积累量可忽略不计。乳酸生成速率在一系列根据CCRG分类确定的白内障严重程度范围内相对一致。测量了培养基中几种干扰物的影响。发现3 mM的ATP浓度可抑制乳酸生成。培养基中标记的6-磷酸葡萄糖产生乳酸的速率约为葡萄糖的一半。葡萄糖浓度升高导致乳酸生成略有减少，并且在一些晶状体中产生少量山梨醇。总体而言，糖酵解途径似乎正常运作，且与皮质和核混浊无关。
