Crawford Michael W, Posch Galen, Cattin-Ortolá Jerôme, Topalidou Irini, Ailion Michael
Department of Biochemistry, University of Washington, Seattle, WA USA.
MicroPubl Biol. 2023 Jul 30;2023. doi: 10.17912/micropub.biology.000918. eCollection 2023.
The NXF-1 : NXT-1 heterodimer is essential for the nuclear export of mRNA. Here we describe three new alleles of and one allele of isolated from a forward genetic screen. These mutations cause no apparent phenotype under normal growth conditions, but partially suppress the lethality caused by heat-shock induced expression of the PEEL-1 toxin from . There is also decreased expression of in an mutant. We propose that NXF-1 : NXT-1 influences the expression of heat-shock activated genes due to a role in the recruitment of the promoter to the nuclear pore complex during heat-shock.
NXF-1:NXT-1异二聚体对于mRNA的核输出至关重要。在此，我们描述了从正向遗传筛选中分离出的三个新的NXF-1等位基因和一个NXT-1等位基因。这些突变在正常生长条件下不会导致明显的表型，但能部分抑制由热休克诱导表达来自嗜线虫致病杆菌的PEEL-1毒素所引起的致死性。在NXF-1突变体中，NXT-1的表达也会降低。我们提出，NXF-1:NXT-1由于在热休克期间将NXT-1启动子募集到核孔复合体中发挥作用，从而影响热休克激活基因的表达。
