Ali Nadia Sufdar, Huang Fang, Qin Wensheng, Yang Trent Chunzhong
Department of Biology, Lakehead University, Thunder Bay, ON, Canada.
Aquatic and Crop Resource Development Research Centre, National Research Council, Ottawa, ON, Canada.
Biotechnol Rep (Amst). 2023 Jul 28;39:e00809. doi: 10.1016/j.btre.2023.e00809. eCollection 2023 Sep.
High throughput screening approaches can significantly speed up the identification of novel enzymes from natural microbial consortiums. A two-step high throughput screening process was proposed and explored to screen lignin-degrading microorganisms. By employing this modified culture enrichment method and screening based on enzyme activity, a total of 82 bacterial and 46 fungal strains were isolated from fifty decayed wood samples (100 liquid cultures) collected from the banks of the Ottawa River in Canada. Among them, ten bacterial and five fungal strains were selected and identified based on their high laccase activities by 16S rDNA and ITS gene sequencing, respectively. The study identified bacterial strains from various genera including , and , along with fungal counterparts including and . Moreover, (AORF21), . (AORF43), (AORF3) and sp. (AORB55) exhibited xylanase and - glucanase activities in addition to laccase production. The proposed approach allowed for the quick identification of promising consortia and enhanced the chance of isolating desired strains based on desired enzyme activities. This method is not limited to lignocellulose and lignin-degrading microorganisms but can be applied to identify novel microbial strains and enzymes from different natural samples.
高通量筛选方法能够显著加快从天然微生物群落中鉴定新型酶的速度。本文提出并探索了一种两步高通量筛选方法来筛选木质素降解微生物。通过采用这种改良的培养富集方法并基于酶活性进行筛选，从加拿大渥太华河岸采集的五十个腐烂木材样本（100个液体培养物）中总共分离出82株细菌和46株真菌。其中，分别基于16S rDNA和ITS基因测序，根据其高漆酶活性选择并鉴定了十株细菌和五株真菌。该研究鉴定出了包括[具体细菌属名未给出]等不同属的细菌菌株，以及包括[具体真菌属名未给出]等的真菌菌株。此外，具体菌株名称未给出、具体菌株名称未给出、具体菌株名称未给出和[具体菌株名称未给出] sp. (AORB55)除了产生漆酶外，还表现出木聚糖酶和β - 葡聚糖酶活性。所提出的方法能够快速鉴定有潜力的群落，并增加了基于所需酶活性分离所需菌株的机会。该方法不仅限于木质纤维素和木质素降解微生物，还可应用于从不同天然样本中鉴定新型微生物菌株和酶。
