Tavakoli Kareshk Amir, Tavakoli Oliaee Razieh, Mahmoudvand Hossein, Keyhani Amir, Mohammadi Mohammad Ali, Bamorovat Mehdi, Hajhosseini Mohammad Ali, Zia-Ali Naser
Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Dept. of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Parasitol. 2018 Jul-Sep;13(3):382-391.
We isolated from camels by bioassay method in mice model and detect parasitic DNA in brain mice by molecular methods.
One hundred tissue samples including heart (n=50), and diaphragm (n=50) were collected from camels (n=50) slaughtered in abattoirs from Feb to Oct 2015 in three provinces located in eastern Iran. In first, blood sample from 50 camels was assayed for anti- antibodies by modified agglutination test (MAT) test. Bioassay method was done in positive MAT blood camels in BALB/c mice and Nested PCR performed in seropositive tissue samples to amplify the B1 and GRA6 genes. The existence of polymorphic restriction sites for endonuclease MseI was used with PCRRFLP method and Sequencing analysis to evaluate the prevalence of type strains (I, II and III).
Overall, 13 (26%) of camels were positive with titer of 1:20 for toxoplasmosis and 13(26%) tissue samples of camels were found positive for the B1 gene, including 7(14%) diaphragm, 6(12%) heart. Moreover, 3(6%) tissue samples of camels were found positive with GRA6 gene for . There are three genotypes and mix genotype using MseI enzyme among all positive samples.
The obtained results from serological and molecular tests demonstrated the infection of with previously recognized genotypes in the tissues of camels for first time from Iran. Since consumption of meat camels are raising in Iran, there may be a high risk of toxoplasmosis through consumption of products from these hosts due to their susceptibility to the infection.
我们通过小鼠模型生物测定法从骆驼中分离，并通过分子方法检测小鼠脑中的寄生虫DNA。
2015年2月至10月期间，在伊朗东部三个省份的屠宰场收集了100份组织样本，包括心脏（n = 50）和膈肌（n = 50），样本来自50头骆驼。首先，通过改良凝集试验（MAT）检测50头骆驼的血液样本中的抗抗体。对BALB/c小鼠中的MAT血阳性骆驼进行生物测定法，并对血清阳性组织样本进行巢式PCR以扩增B1和GRA6基因。使用PCR-RFLP方法和测序分析，利用核酸内切酶MseI的多态性限制位点来评估I、II和III型菌株的流行情况。
总体而言，13头（26%）骆驼弓形虫病检测呈阳性，滴度为1:20，13份（26%）骆驼组织样本的B1基因检测呈阳性，其中7份（14%）来自膈肌，6份（12%）来自心脏。此外，3份（6%）骆驼组织样本的GRA6基因检测呈阳性。在所有阳性样本中，使用MseI酶检测到三种基因型和混合基因型。
血清学和分子检测结果首次证明伊朗骆驼组织中存在先前已确认基因型的弓形虫感染。由于伊朗食用骆驼肉的情况不断增加，这些宿主易受感染，因此食用其产品可能存在较高的弓形虫病风险。
