Crofts Fraser, Al-Majali Ahmad, Gerring David, Gubbins Simon, Hicks Hayley, Campbell Dana, Wilson Steve, Chesang Lizzie, Stuke Kristin, Cordel Claudia, Parida Satya, Batten Carrie
The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, United Kingdom.
Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan.
Vaccine X. 2023 Aug 4;15:100363. doi: 10.1016/j.jvacx.2023.100363. eCollection 2023 Dec.
A novel liquid stabiliser was tested with the Nigeria 75/1 Peste des Petit Ruminants (PPR) vaccine over two field studies carried out in sheep and goats. PPR seronegative sheep and goats were selected from farms surrounding Amman, Jordan and were vaccinated with either a stabilised liquid PPR vaccine that had been formulated 3 months prior to use and stored at 2-8 °C or a reconstituted lyophilised PPRV vaccine reconstituted on the day of vaccination. Sera were taken immediately before vaccination and at approximately 1.5, 3 and 6 months following vaccination, then subsequently tested using IDVet ID Screen® PPR competition ELISA and Serum Neutralisation tests to determine the presence of PPRV anti-N antibodies and neutralising antibodies, respectively. It was observed that the liquid-stabilised vaccine was able to provide comparable antibody responses in both species to those induced by the lyophilized vaccine. The ability to store liquid stabilised PPRV vaccine for field use would positively impact PPRV eradication efforts.
在对绵羊和山羊进行的两项实地研究中，一种新型液体稳定剂与尼日利亚75/1型小反刍兽疫（PPR）疫苗一同进行了测试。PPR血清阴性的绵羊和山羊从约旦安曼周边的农场中挑选出来，分别接种使用前3个月配制并储存于2-8°C的稳定化液体PPR疫苗，或在接种当天重构的冻干PPRV疫苗。在接种前以及接种后约1.5、3和6个月采集血清，随后分别使用IDVet ID Screen® PPR竞争ELISA和血清中和试验进行检测，以确定PPRV抗N抗体和中和抗体的存在情况。结果观察到，液体稳定化疫苗在这两个物种中能够产生与冻干疫苗诱导的抗体反应相当的抗体反应。储存液体稳定化PPRV疫苗以供实地使用的能力将对PPRV根除工作产生积极影响。
