Qilu Hospital of Shandong University, Jinan, China.
QiLu University of Technology, Jinan, China.
PeerJ. 2023 Aug 11;11:e15829. doi: 10.7717/peerj.15829. eCollection 2023.
Oxidative stress refers to the imbalance between oxidants and antioxidants in organisms and often induces hepatic inflammation. Supplementing exogenous superoxide dismutase is an effective way to alleviate oxidative stress; however, the effects and mechanisms by which superoxide dismutase alleviates hepatic inflammation remain unclear.
This study established a Kunming mouse model to verify and investigate the oxidative stress and hepatic inflammation-alleviating effects of the superoxide dismutase oral supplement that was prepared by our research group in a previous study.
The superoxide dismutase product significantly restored the body weight and liver alanine transaminase, aspartate aminotransferase, superoxide dismutase, catalase, glutathione, and glutathione peroxidase levels of oxidative stress induced mice. Moreover, exogenous superoxide dismutase significantly inhibited interleukin 1 and interleukin 6 mRNA expression in the livers of mice with hepatic inflammation. Transcriptomic analysis indicated that superoxide dismutase had a significant inhibitory effect on expression, alleviating oxidative stress damage, and mediating liver cell apoptosis by regulating the expression of , , and .
Our research verified the oxidative stress remediation effects of superoxide dismutase and its therapeutic role against hepatic inflammation. This study can lay a foundation for investigating the mechanism by which superoxide dismutase alleviates hepatic disease.
氧化应激是指生物体内氧化剂和抗氧化剂之间的失衡，常导致肝炎症。补充外源性超氧化物歧化酶是缓解氧化应激的有效方法，但超氧化物歧化酶缓解肝炎症的作用和机制尚不清楚。
本研究建立了昆明小鼠模型，验证和研究了本研究组前期研究制备的超氧化物歧化酶口服补充剂对氧化应激和肝炎症的缓解作用。
超氧化物歧化酶产品显著恢复了氧化应激诱导的小鼠体重和肝丙氨酸转氨酶、天冬氨酸转氨酶、超氧化物歧化酶、过氧化氢酶、谷胱甘肽和谷胱甘肽过氧化物酶水平。此外，外源性超氧化物歧化酶显著抑制了肝炎症小鼠肝脏中白细胞介素 1 和白细胞介素 6 mRNA 的表达。转录组分析表明，超氧化物歧化酶通过调节 、 、 和 的表达，对 表达具有显著抑制作用，缓解氧化应激损伤，介导肝细胞凋亡。
本研究验证了超氧化物歧化酶的氧化应激修复作用及其对肝炎症的治疗作用。该研究可为研究超氧化物歧化酶缓解肝疾病的机制奠定基础。
