[Identification and expression pattern analysis of gene family in castor under abiotic stresses].

Auto-inhibited Ca-ATPase (ACA) is one of the Ca-ATPase subfamilies that plays an important role in maintaining Ca concentration balance in plant cells. To explore the function and gene expression pattern of the gene family in castor, bioinformatics analysis was used to identify the members of the gene family in castor. The basic physical and chemical properties, subcellular location, protein secondary and tertiary structure, conserved domain, conserved motif, gene structure, chromosome location and collinear relationship, as well as the evolutionary characteristics and promoter -acting elements were predicted and analyzed. The expression pattern of the gene under abiotic stress was analyzed by expression (fragments per kilobase of exon model per million mapped fragments, FPKM) in castor transcriptome data. The results showed that 8 gene family members were identified in castor, acidic proteins located in the plasma membrane. In the secondary structure of all proteins, the α-helix and random coil is more; the genes were clustered into three categories, and the design of the genes in the same category was similar to the conserved motif. Both of them had four typical domains, - had a Ca-ATPase N-terminal autoinhibitory domain. The gene is mostly located on the long arm of the chromosome and has 2 pairs of collinear relationships. There are more light response elements but fewer hormone-induced elements located upstream of the coding region. Interspecific clustering showed that the evolution of genes among species was conservative. Tissue expression pattern analysis showed that genes showed apparent tissue expression specificity, and most of the genes showed the highest expression level in male flowers. Expression analysis under abiotic stress showed that - were up-regulated under high salt and drought stress, and was up-regulated at 0-24 h under low-temperature stress, indicating that genes positively responded to abiotic stresses. The above results provide a theoretical basis for exploring the role of the gene in castor growth, development and stress response.