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[]的叶绿体基因组特征与系统发育分析

[Chloroplast genomic characterization and phylogenetic analysis of ].

作者信息

Yan Li, Yang Xuelian, Wu Yongfei, Wang Xia, Hu Xiaojing

机构信息

Department of Horticulture, College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2023 Jul 25;39(7):2914-2925. doi: 10.13345/j.cjb.220830.

Abstract

belongs to the genus in the family of , and is a high-quality wild vegetables with high nutritional value. In this study, high-throughput techniques were used to sequence, assemble and annotate the chloroplast genome. We also analyzed its structure, and construct the phylogenetic trees from the . to further study the chloroplast genome characteristics. The results showed that the chloroplast genome size was 153 220 bp, and the GC content was 36.4%, which belonged to the typical tetrad structure in . . The chloroplast genome encodes 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes in . . Among them, 15 genes contained 1 intron, 2 genes contained 2 introns, and had trans-splicing, respectively. In . , chloroplast genomes could be divided into four categories, including 43 photosynthesis, 64 self-replication, other 7 coding proteins, and 4 unknown functions. A total of 51 073 codons were detected in the chloroplast genome, among which the codon encoding leucine (Leu) accounted for the largest proportion, and the codon preferred to use A and U bases. There were 72 simple sequence repeats (SSRs) in the chloroplast genome of . , containing 58 single nucleotides, 12 dinucleotides, 1 trinucleotide, and 1 tetranucleotide. The 1 gene expansion was present at the IRb/SSC boundary. The phylogenetic trees showed that . (OL800583) was most closely related to (MZ292972), (MK227819) and var. (MN189961). Taken together, our results provide worthwhile information for understanding the identification, genetic evolution, and genomics research of . species.

摘要

属于科中的属,是一种具有高营养价值的优质野生蔬菜。在本研究中,采用高通量技术对叶绿体基因组进行测序、组装和注释。我们还分析了其结构,并从……构建系统发育树,以进一步研究叶绿体基因组特征。结果表明,叶绿体基因组大小为153220 bp,GC含量为36.4%,属于……中的典型四分体结构。叶绿体基因组编码130个基因,包括85个蛋白质编码基因、37个tRNA基因和8个rRNA基因。其中,15个基因含有1个内含子,2个基因含有2个内含子,……分别具有反式剪接。在……中,叶绿体基因组可分为四类,包括43个光合作用相关基因、64个自我复制相关基因、其他7个编码蛋白质基因和4个功能未知基因。叶绿体基因组中共检测到51073个密码子,其中编码亮氨酸(Leu)的密码子占比最大,且密码子偏好使用A和U碱基。……的叶绿体基因组中有72个简单序列重复(SSR),包括58个单核苷酸、12个二核苷酸、1个三核苷酸和1个四核苷酸。在IRb/SSC边界存在1个基因扩张。系统发育树表明,……(OL800583)与……(MZ292972)、……(MK227819)和……变种……(MN189961)关系最为密切。综上所述,我们的结果为理解……物种的鉴定、遗传进化和基因组学研究提供了有价值的信息。

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