Zhang Jin, Deng Yongbiao, Zhao Bo
Department of Pharmacognosy, Pharmacy School, Guilin Medical University, Guilin 541199, Guangxi, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Jan 25;40(1):280-291. doi: 10.13345/j.cjb.230160.
In this study, the chloroplast genome of Orel & Curry was sequenced using high-throughput sequencing technology. The results showed that the chloroplast genome of . was 156 882 bp in length with a typical tetrad structure, encoding 132 genes, including 88 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. Codon preference analysis revealed that the highest number of codons coded for leucine, with a high A/U preference in the third codon position. Additionally, 67 simple sequence repeats (SSR) loci were identified, with a preference for A and T bases. The inverted repeat (IR) boundary regions of the chloroplast genome of . were relatively conserved, except for a few variable regions. Phylogenetic analysis indicated that . was most closely related to . . Yellow is a valuable material for genetic engineering breeding. This study provides fundamental genetic information on chloroplast engineering and offers valuable resources for conducting in-depth research on the evolution, species identification, and genomic breeding of yellow .
在本研究中，利用高通量测序技术对奥雷尔和库里（Orel & Curry）的叶绿体基因组进行了测序。结果表明，[此处原文缺失具体物种名称]的叶绿体基因组长度为156882 bp，具有典型的四分体结构，编码132个基因，包括88个蛋白质编码基因、36个tRNA基因和8个rRNA基因。密码子偏好性分析显示，编码亮氨酸的密码子数量最多，在密码子的第三个位置具有较高的A/U偏好性。此外，还鉴定出67个简单序列重复（SSR）位点，对A和T碱基有偏好。[此处原文缺失具体物种名称]叶绿体基因组的反向重复（IR）边界区域相对保守，除了少数可变区域。系统发育分析表明，[此处原文缺失具体物种名称]与[此处原文缺失具体物种名称]关系最为密切。[此处原文缺失具体物种名称]是基因工程育种的宝贵材料。本研究提供了关于叶绿体工程的基础遗传信息，并为深入研究[此处原文缺失具体物种名称]的进化、物种鉴定和基因组育种提供了宝贵资源。
