Wu Yongfei, Yang Xuelian, Wang Xia, Yan Li, Zhang Wanping
College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, China.
Vegetable Research Academy, Guizhou University, Guiyang 550025, Guizhou, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Jul 25;39(7):2939-2953. doi: 10.13345/j.cjb.220831.
The genomic DNA of was extracted and sequenced by Illumina NovaSeq platform to obtain the complete chloroplast genome sequence, and the sequence characteristics and phylogenetic analysis of chloroplast genes were carried out. The results showed that the complete chloroplast genome of the . was 155 650 bp in length and had a typical tetrad structure, including two reverse repeats (25 748 bp each), a large copy region (85 443 bp) and a small copy region (18 711 bp). A total of 131 genes were identified in the whole genome of . chloroplast, including 86 protein coding genes, 37 tRNA genes and 8 rRNA genes. The GC content of the whole genome was 36.9%. The genome of . chloroplast contains 47 scattered repeats and 72 simple sequence repeating (SSR) loci. The codon preference is leucine codon, and the codon at the end of A/U is preferred. Phylogenetic analysis showed that . had the closest relationship with . , followed by . and . . The chloroplast genome characteristics and phylogenetic analysis of . provide a theoretical basis for its genetic diversity research and chloroplast development and utilization.
提取了[物种名称]的基因组DNA，并通过Illumina NovaSeq平台进行测序以获得完整的叶绿体基因组序列，同时对叶绿体基因的序列特征和系统发育进行了分析。结果表明，[物种名称]的完整叶绿体基因组长度为155650 bp，具有典型的四分体结构，包括两个反向重复序列（各25748 bp）、一个大单拷贝区域（85443 bp）和一个小单拷贝区域（18711 bp）。在[物种名称]叶绿体的全基因组中共鉴定出131个基因，包括86个蛋白质编码基因、37个tRNA基因和8个rRNA基因。全基因组的GC含量为36.9%。[物种名称]叶绿体基因组包含47个散在重复序列和72个简单序列重复（SSR）位点。密码子偏好为亮氨酸密码子，且偏好以A/U结尾的密码子。系统发育分析表明，[物种名称]与[物种名称]关系最为密切，其次是[物种名称]和[物种名称]。[物种名称]的叶绿体基因组特征和系统发育分析为其遗传多样性研究以及叶绿体的开发利用提供了理论依据。
