Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
University Hospital and Center for Biomedicine and Innovations, Faculty of Medicine, Macau University of Science and Technology, Taipa 999078, Macau, China; Zhuhai International Eye Center, Zhuhai People's Hospital and the First Affiliated Hospital of Faculty of Medicine, Macau University of Technology, Zhuhai, Guangdong, China; Department of Bioinformatics and AI, Guangzhou Laboratory, Guangzhou, China; School of Medicine, University of Dundee, Dundee, UK.
Cell Rep Med. 2023 Aug 15;4(8):101156. doi: 10.1016/j.xcrm.2023.101156.
We describe a general approach to produce bone and cartilaginous structures utilizing the self-regenerative capacity of the intercostal rib space to treat a deformed metacarpophalangeal joint and microtia. Anatomically precise 3D molds were positioned on the perichondro-periosteal or perichondral flap of the intercostal rib without any other exogenous elements. We find anatomically precise metacarpal head and auricle constructs within the implanted molds after 6 months. The regenerated metacarpal head was used successfully to surgically repair the deformed metacarpophalangeal joint. Auricle reconstructive surgery in five unilateral microtia patients yielded good aesthetic and functional results. Long-term follow-up revealed the auricle constructs were safe and stable. Single-cell RNA sequencing analysis reveal early infiltration of a cell population consistent with mesenchymal stem cells, followed by IL-8-stimulated differentiation into chondrocytes. Our results demonstrate the repair and regeneration of tissues using only endogenous factors and a viable treatment strategy for bone and tissue structural defects.
我们描述了一种利用肋间肋骨空间的自我再生能力来治疗变形的掌指关节和小耳畸形的通用方法。在没有任何其他外源性元素的情况下，将解剖精确的 3D 模具定位在肋间肋软骨或肋软骨瓣上。我们在植入的模具中发现了 6 个月后具有解剖精确的掌骨头和耳廓结构。再生的掌骨头成功地用于手术修复变形的掌指关节。5 例单侧小耳畸形患者的耳廓重建手术获得了良好的美学和功能效果。长期随访显示，耳廓结构安全稳定。单细胞 RNA 测序分析显示，早期有一群细胞浸润，与间充质干细胞一致，随后受到 IL-8 刺激分化为软骨细胞。我们的结果表明，仅使用内源性因子修复和再生组织是可行的，为骨和组织结构缺陷提供了一种可行的治疗策略。
