一种新的小鼠α干扰素基因：MuIFN-α I9的结构与表达

Structure and expression of a new murine interferon-alpha gene: MuIFN-alpha I9.

作者信息

Seif I, De Maeyer-Guignard J

出版信息

Gene. 1986;43(1-2):111-21. doi: 10.1016/0378-1119(86)90014-4.

Abstract

A new murine alpha interferon gene, MuIFN-alpha I9, isolated from a BALB/c genomic clone, was characterized. It encodes a mature polypeptide of 167 amino acids (aa), presenting from 77 to 86% homology with the seven other MuIFN-alpha I aa sequences previously described. When compared to the latter, pre-IFN-alpha I9 has 13 distinctive aa, and, remarkably, ten of these occur in pairs. The coding region, fused to the SV40 early promoter and introduced into COS monkey cells, directed the transient secretion of an acid-stable functional IFN of 18-21 kDa. The production in this system reached levels of 300 000 units per 0.15 ml. A comparison of the aa sequence of different murine, rat, bovine, and human alpha and beta IFNs revealed certain common features allowing us to propose a putative secondary structure of the IFN proteins. A detailed analysis of results previously published by us and by others showed that the MuIFN-alpha I9 gene is, together with a least twelve other MuIFN-alpha I genes, located on chromosome 4.

摘要

从BALB/c基因组克隆中分离出一种新的小鼠α干扰素基因MuIFN-α I9，并对其进行了表征。它编码一个由167个氨基酸（aa）组成的成熟多肽，与先前描述的其他七个MuIFN-α I氨基酸序列具有77%至86%的同源性。与后者相比，前体IFN-α I9有13个独特的氨基酸，其中十个以成对形式出现。编码区与SV40早期启动子融合，并导入COS猴细胞，指导瞬时分泌一种18 - 21 kDa的酸稳定功能性干扰素。该系统中的产量达到每0.15 ml 300000单位。对不同小鼠、大鼠、牛和人α及β干扰素的氨基酸序列比较揭示了某些共同特征，使我们能够提出干扰素蛋白的假定二级结构。对我们和其他人先前发表的结果进行详细分析表明，MuIFN-α I9基因与至少其他十二个MuIFN-α I基因一起位于4号染色体上。