Kelley K A, Kozak C A, Dandoy F, Sor F, Skup D, Windass J D, DeMaeyer-Guignard J, Pitha P M, DeMaeyer E
Gene. 1983 Dec;26(2-3):181-8. doi: 10.1016/0378-1119(83)90188-9.
A cDNA library was constructed from polysomal poly(A)+RNA from Newcastle disease virus (NDV)-induced mouse C243 cells, and screened with a human interferon-alpha (HuIFN-alpha) cDNA probe. A cDNA clone for one of the murine interferon-alpha (MuIFN-alpha) genes was isolated, and sequencing analysis revealed that it was a partial copy which is almost identical to the published sequence for the MuIFN-alpha 2 gene. This partial cDNA clone represents a virus-induced message as seen by Northern blot analysis of RNA from NDV-induced C243 cells, and Southern blot analysis of DNA from BALB/c mouse revealed the presence of a multiple IFN-alpha gene family. The MuIFN-alpha genes were mapped to chromosome 4 by Southern blot analysis of hamster/mouse somatic cell hybrid DNAs.
从新城疫病毒（NDV）诱导的小鼠C243细胞的多聚核糖体聚腺苷酸（poly(A)）+RNA构建了一个cDNA文库，并用人类α干扰素（HuIFN-α）cDNA探针进行筛选。分离出了一个鼠α干扰素（MuIFN-α）基因的cDNA克隆，测序分析表明它是一个部分拷贝，与已发表的MuIFN-α 2基因序列几乎相同。通过对NDV诱导的C243细胞RNA的Northern印迹分析可以看出，这个部分cDNA克隆代表了一种病毒诱导的信息，而对BALB/c小鼠DNA的Southern印迹分析显示存在一个多干扰素-α基因家族。通过对仓鼠/小鼠体细胞杂交DNA的Southern印迹分析，将MuIFN-α基因定位到了4号染色体上。
