新玫瑰单胞菌（Neoroseomonas alba）sp. nov.、硝酸还原玫瑰单胞菌（Neoroseomonas nitratireducens）sp. nov.、吲哚产生栖稻单胞菌（Paraoseomonas indoligenes）sp. nov. 和杆状栖稻单胞菌（Paraoseomonas baculiformis）sp. nov.，均从稻田根际土壤中分离得到。

Neoroseomonas alba sp. nov., Neoroseomonas nitratireducens sp. nov., Paraoseomonas indoligenes sp. nov and Paraoseomonas baculiformis sp. nov., isolated from the rhizosphere of paddy soil.

机构信息

Department of Life Science, Dongguk University Seoul, Goyang, 10326, South Korea.

出版信息

Antonie Van Leeuwenhoek. 2023 Oct;116(10):1009-1022. doi: 10.1007/s10482-023-01863-8. Epub 2023 Aug 16.

Abstract

Four novel Gram-stain negative bacteria, designated as HAJ6, PWR1, SG15 and SSH11, were isolated from the soil sample of paddy fields from Goyang in the Republic of Korea. The isolated strains were aerobic, short-rod or rod shaped, non-sporulating. They grew optimally at 30 °C, pH 7 and without additional NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that they belong to the genus of Neoroseomonas and Pararoseomonas and closely relate to Neoroseomonas terrae DS-48 (97.5%; HJA6), Neoroseomonas rubea MO17 (99.4%; PWR1), Pararoseomonas pecuniae N75 (97.3%; SG15) and Pararoseomonas rosea 173-96 (97.8%; SSH11). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of the isolates with the most closely related strains were 72.9-90.9% and 19.1-42.5%, respectively. The major fatty acids in the isolates were C, C cyclo ω8c, C 2-OH and summed feature 8 (composed of C ω7c and/or C ω6c), and the predominant quinone was ubiquinone 10. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, and other unidentified polar lipids. Based on the draft genome sequences, the genomic DNA G + C content of HAJ6, PWR1, SG15 and SSH11 were 69.5%, 72.0%, 70.8% and 69.7%, respectively. All isolates produced indole-3-acetic acid (IAA), a type of plant growth hormone in the presence of -tryptophan. Physiological and biochemical tests and 16S rRNA sequence analysis clearly revealed that the isolates were novel species belonging to the genus Neoroseomonas and Pararoseomonas. Their proposed names were as follows: Neoroseomonas alba sp. nov. for strain HJA6 (= KACC 21545 = NBRC 114316), Neoroseomonas nitratireducens sp. nov. for strain PWR1 (= KCTC 82687 = NBRC 114490), Pararoseomonas indoligenes sp. nov. for strain SG15 (= KCTC 82686 = NBRC 114481) and Paraoseomonas baculiformis sp. nov. for strain SSH11 (= KCTC 82685 = NBRC 11482).

摘要

从韩国高阳稻田土壤样本中分离到四株革兰氏阴性细菌，分别命名为 HAJ6、PWR1、SG15 和 SSH11。分离株为需氧、短杆或杆状、不产孢。它们在 30°C、pH7 且无需额外 NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，它们属于 Neoroseomonas 和 Pararoseomonas 属，与 Neoroseomonas terrae DS-48（97.5%；HJA6）、Neoroseomonas rubea MO17（99.4%；PWR1）、Pararoseomonas pecuniae N75（97.3%；SG15）和 Pararoseomonas rosea 173-96（97.8%；SSH11）密切相关。与最密切相关的菌株相比，分离株的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 72.9-90.9%和 19.1-42.5%。分离株的主要脂肪酸为 C、C 环 ω8c、C 2-OH 和特征性 8（由 C ω7c 和/或 C ω6c 组成），主要醌为泛醌 10。极性脂谱由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和其他未鉴定的极性脂组成。基于基因组草图序列，HAJ6、PWR1、SG15 和 SSH11 的基因组 DNA G+C 含量分别为 69.5%、72.0%、70.8%和 69.7%。所有分离株在存在 -色氨酸的情况下均能产生吲哚-3-乙酸（IAA），这是一种植物生长激素。生理生化试验和 16S rRNA 序列分析清楚地表明，这些分离株是属于 Neoroseomonas 和 Pararoseomonas 属的新种。它们的暂定名称如下：HJA6（=KACC 21545= NBRC 114316）为 Neoroseomonas alba sp. nov.，PWR1（=KCTC 82687= NBRC 114490）为 Neoroseomonas nitratireducens sp. nov.，SG15（=KCTC 82686= NBRC 114481）为 Pararoseomonas indoligenes sp. nov.，SSH11（=KCTC 82685= NBRC 11482）为 Paraoseomonas baculiformis sp. nov.。