Chen Xiao-Fang, Chen Xiao-Qing, Luo Hai-Lian, Xia Li-Na, Huang Shu-Hui, Chen Qi
Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Nanchang University, Nanchang, 330000, Jiangxi, China.
Department of Obstetrics and Gynecology, Jiangxi Maternal and Child Health Hospital, Nanchang, 330000, Jiangxi, China.
Proteome Sci. 2023 Aug 16;21(1):12. doi: 10.1186/s12953-023-00213-w.
In this study, we aimed to identify differentially expressed heat shock protein (HSP) profiles in the villi and decidua from patients with early missed abortion (EMA).
By using high-throughput and high-precision parallel reaction monitoring (PRM)-based targeted proteomics techniques, this study examined the abundance of HSPs in the villi and decidua of 10 patients with EMA and 10 controls. Moreover, the abundance of 3 HSPs in the villi of another 22 patients with EMA and 22 controls was verified with Western blotting and immunohistochemistry (IHC).
There were potential differences in the abundance of 16 HSPs and 42 polypeptides in human villi and decidua compared with those of the control group. Among them, HSP90AB1, HSPD1 and HSPA13 were downregulated in abundance in villi of patients with EMA, with a statistically significant difference, which was consistent with the verification results of Western blots and IHC.
Using a PRM-based targeted proteomics technique, this study is the first to screen and quantitatively analyze the expression profile of HSPs in the villi and decidua of patients with EMA. The significant downregulation of HSP90AB1, HSPD1 and HSPA13 was found to have a potentially intimate association with the occurrence of EMA. The findings in our study may provide novel potential research targets related to HSPs for the pathogenesis, prevention and treatment of EMA.
在本研究中，我们旨在鉴定早期稽留流产（EMA）患者绒毛和蜕膜中差异表达的热休克蛋白（HSP）谱。
本研究采用基于高通量和高精度平行反应监测（PRM）的靶向蛋白质组学技术，检测了10例EMA患者和10例对照者绒毛和蜕膜中HSP的丰度。此外，通过蛋白质免疫印迹法和免疫组织化学（IHC）对另外22例EMA患者和22例对照者绒毛中3种HSP的丰度进行了验证。
与对照组相比，人绒毛和蜕膜中16种HSP和42种多肽的丰度存在潜在差异。其中，EMA患者绒毛中HSP90AB1、HSPD1和HSPA13的丰度下调，差异具有统计学意义，这与蛋白质免疫印迹法和免疫组织化学的验证结果一致。
本研究首次利用基于PRM的靶向蛋白质组学技术筛选并定量分析了EMA患者绒毛和蜕膜中HSP的表达谱。发现HSP90AB1、HSPD1和HSPA13的显著下调可能与EMA的发生密切相关。本研究结果可能为EMA的发病机制、预防和治疗提供与HSP相关的新的潜在研究靶点。
