Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand.
PLoS One. 2023 Aug 17;18(8):e0289733. doi: 10.1371/journal.pone.0289733. eCollection 2023.
Bacterial content of mosquitoes has given rise to the development of innovative tools that influence and seek to control malaria transmission. This study identified the bacterial microbiota in field-collected female adults of the Anopheles hyrcanus group and three Anopheles species, Anopheles nivipes, Anopheles philippinensis, and Anopheles vagus, from an endemic area in the southeastern part of Ubon Ratchathani Province, northeastern Thailand, near the Lao PDR-Cambodia-Thailand border. A total of 17 DNA libraries were generated from pooled female Anopheles abdomen samples (10 abdomens/ sample). The mosquito microbiota was characterized through the analysis of DNA sequences from the V3-V4 regions of the 16S rRNA gene, and data were analyzed in QIIME2. A total of 3,442 bacterial ASVs were obtained, revealing differences in the microbiota both within the same species/group and between different species/group. Statistical difference in alpha diversity was observed between An. hyrcanus group and An. vagus and between An. nivipes and An. vagus, and beta diversity analyses showed that the bacterial community of An. vagus was the most dissimilar from other species. The most abundant bacteria belonged to the Proteobacteria phylum (48%-75%) in which Pseudomonas, Serratia, and Pantoea were predominant genera among four Anopheles species/group. However, the most significantly abundant genus observed in each Anopheles species/group was as follows: Staphylococcus in the An. hyrcanus group, Pantoea in the An. nivipes, Rosenbergiella in An. philippinensis, and Pseudomonas in An. vagus. Particularly, Pseudomonas sp. was highly abundant in all Anopheles species except An. nivipes. The present study provides the first study on the microbiota of four potential malaria vectors as a starting step towards understanding the role of the microbiota on mosquito biology and ultimately the development of potential tools for malaria control.
蚊子的细菌含量催生了一系列创新工具，这些工具影响并试图控制疟疾的传播。本研究从泰国东北部乌汶叻差他尼府东南部一个流行地区采集的野外雌性成蚊中鉴定了按蚊属和三种按蚊（阿蚊、微小按蚊和淡色库蚊）的细菌微生物群。从 10 只雌性按蚊腹部样本（每个样本 10 只腹部）中总共生成了 17 个 DNA 文库。通过分析 16S rRNA 基因 V3-V4 区的 DNA 序列来描述蚊子的微生物群，使用 QIIME2 对数据进行分析。共获得 3442 个细菌 ASV，揭示了同一物种/组内和不同物种/组间微生物群的差异。在安蚊属组和淡色库蚊之间以及微小按蚊和淡色库蚊之间观察到 alpha 多样性的统计学差异，beta 多样性分析表明，淡色库蚊的细菌群落与其他物种最不相似。最丰富的细菌属于变形菌门（48%-75%），其中假单胞菌、沙雷氏菌和泛菌是四个按蚊物种/组中的主要属。然而，在每个按蚊物种/组中观察到的最丰富的属如下：在安蚊属组中为葡萄球菌属，在微小按蚊中为泛菌属，在菲律宾按蚊中为罗森贝格氏菌属，在淡色库蚊中为假单胞菌属。特别地，假单胞菌属在除微小按蚊外的所有按蚊种中都高度丰富。本研究首次对四种潜在疟疾传播媒介的微生物群进行了研究，这是了解微生物群对蚊子生物学作用的起点，并最终为开发疟疾控制的潜在工具奠定了基础。
