Department of Medical Entomology, Centre for Research in Infectious Diseases (CRID), LSTM Research Unit, Yaoundé, Cameroon.
Department of Microbiology and Infectious Diseases, School of Veterinary Medicine and Sciences, University of Ngaoundéré, Ngaoundéré, Cameroon.
Med Vet Entomol. 2022 Sep;36(3):269-282. doi: 10.1111/mve.12583. Epub 2022 May 17.
Understanding the environmental factors affecting the microbiota in malaria vectors may help in the development of novel vector control interventions, similar to paratransgenesis. This study evaluated seasonal and geographical variations in the microbial community of the two major malaria vectors. Adult Anopheles mosquitoes were collected across two different eco-geographical settings in Cameroon, during the dry and wet seasons. DNA was extracted from the whole individual mosquitoes from each group and processed for microbial analysis using Illumina Miseq sequencing of the V3-V4 region of the 16S rRNA gene. Data analysis was performed using QIIME2 and R software programs. A total of 1985 mosquitoes were collected and among them, 120 were selected randomly corresponding to 30 mosquitoes per season and locality. Overall, 97 bacterial taxa were detected across all mosquito samples, with 86 of these shared between dry and wet seasons in both localities and species. There were significant differences in bacterial composition between both seasons, with a clear separation observed between the dry and wet seasons (PERMANOVA comparisons of beta diversity, Pseudo-F = 10.45; q-value = 0.01). This study highlights the influence of seasonal variation on microbial communities and this variation's impact on mosquito biology and vectorial capacity should be further investigated.
了解影响疟疾传播媒介微生物群的环境因素，可能有助于类似于共生体转化的新型媒介控制干预措施的发展。本研究评估了喀麦隆两个不同生态地理环境中两种主要疟疾传播媒介的微生物群落的季节性和地理变化。在旱季和雨季，从每个组的整个个体蚊子中提取 DNA，并使用 Illumina Miseq 对 16S rRNA 基因的 V3-V4 区进行测序，进行微生物分析。使用 QIIME2 和 R 软件程序进行数据分析。共收集了 1985 只蚊子，其中随机选择了 120 只，每个季节和地点各 30 只。总体而言，在所有蚊子样本中检测到了 97 种细菌类群，其中 86 种在两个地点的旱季和雨季均有发现。两个季节之间的细菌组成存在显著差异，旱季和雨季之间有明显的分离（PERMANOVA 比较β多样性，Pseudo-F = 10.45；q 值 = 0.01）。本研究强调了季节性变化对微生物群落的影响，应进一步研究这种变化对蚊子生物学和媒介能力的影响。
