Fofana Aminata, Gendrin Mathilde, Romoli Ottavia, Yarbanga G Armel Bienvenu, Ouédraogo Georges Anicet, Yerbanga Rakiswende Serge, Ouédraogo Jean-Bosco
Institut de Recherche en Sciences de la Santé, Bobo Dioulasso, Burkina Faso.
Université Nazi Boni, Bobo-Dioulasso 1091, Burkina Faso.
iScience. 2021 Nov 9;24(12):103416. doi: 10.1016/j.isci.2021.103416. eCollection 2021 Dec 17.
The microbiota of mosquitoes influences malaria transmission. Antibiotics ingested during a blood meal impact the mosquito microbiome and malaria transmission, with substantial differences between drugs. Here, we assessed if amoxicillin affects the gut mosquito microbiota We collected larvae in Burkina Faso, kept them in semi-field conditions, and offered a blood meal to adult females. We tested the impact of blood supplementation with two alternative amoxicillin preparations on microbiota composition, determined by high-throughput sequencing in individual gut samples. Our analysis detected four major genera, , , and . The antibiotic treatment significantly affected overall microbiota composition, with a specific decrease in the relative abundance of and during blood digestion. Besides its interest on the influence of amoxicillin on the mosquito microbiota, our study proposes a thorough approach to report negative-control data of high-throughput sequencing studies on samples with a reduced microbial load.
蚊子的微生物群会影响疟疾传播。在吸食血液期间摄入的抗生素会影响蚊子的微生物组和疟疾传播，不同药物之间存在显著差异。在这里，我们评估了阿莫西林是否会影响蚊子肠道微生物群。我们在布基纳法索收集幼虫，将它们置于半野外条件下，并为成年雌性蚊子提供血液。我们测试了用两种替代阿莫西林制剂补充血液对微生物群组成的影响，通过对个体肠道样本进行高通量测序来确定。我们的分析检测到四个主要属，即 、 、 和 。抗生素处理显著影响了整体微生物群组成，在血液消化过程中， 和 的相对丰度有特定下降。除了对阿莫西林对蚊子微生物群的影响有研究意义外，我们的研究还提出了一种全面的方法来报告关于微生物负荷降低的样本的高通量测序研究的阴性对照数据。
