Dai Shixiang, Harnisch Falk, Morejón Micjel Chávez, Keller Nina Sophie, Korth Benjamin, Vogt Carsten
Department of Environmental Microbiology, Helmholtz Centre for Environmental Research GmbH - UFZ, Leipzig, Germany.
Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research GmbH - UFZ, Leipzig, Germany.
Environ Sci Ecotechnol. 2023 Jul 26;17:100307. doi: 10.1016/j.ese.2023.100307. eCollection 2024 Jan.
Microbial electrochemical technologies have been extensively employed for phenol removal. Yet, previous research has yielded inconsistent results, leaving uncertainties regarding the feasibility of phenol degradation under strictly anaerobic conditions using anodes as sole terminal electron acceptors. In this study, we employed high-performance liquid chromatography and gas chromatography-mass spectrometry to investigate the anaerobic phenol degradation pathway. Our findings provide robust evidence for the purely anaerobic degradation of phenol, as we identified benzoic acid, 4-hydroxybenzoic acid, glutaric acid, and other metabolites of this pathway. Notably, no typical intermediates of the aerobic phenol degradation pathway were detected. One-chamber reactors (+0.4 V vs. SHE) exhibited a phenol removal rate of 3.5 ± 0.2 mg L d, while two-chamber reactors showed 3.6 ± 0.1 and 2.6 ± 0.9 mg L d at anode potentials of +0.4 and + 0.2 V, respectively. Our results also suggest that the reactor configuration certainly influenced the microbial community, presumably leading to different ratios of phenol consumers and microorganisms feeding on degradation products.
微生物电化学技术已被广泛用于去除苯酚。然而，先前的研究结果并不一致，对于在严格厌氧条件下以阳极作为唯一终端电子受体时苯酚降解的可行性仍存在不确定性。在本研究中，我们采用高效液相色谱和气相色谱 - 质谱联用技术来研究厌氧苯酚降解途径。我们的研究结果为苯酚的纯厌氧降解提供了有力证据，因为我们鉴定出了苯甲酸、4 - 羟基苯甲酸、戊二酸以及该途径的其他代谢产物。值得注意的是，未检测到好氧苯酚降解途径的典型中间体。单室反应器（相对于标准氢电极电位为 +0.4 V）的苯酚去除率为3.5±0.2 mg L⁻¹ d⁻¹，而双室反应器在阳极电位分别为 +0.4 V和 +0.2 V时，苯酚去除率分别为3.6±0.1 mg L⁻¹ d⁻¹和2.6±0.9 mg L⁻¹ d⁻¹。我们的结果还表明，反应器配置肯定会影响微生物群落，可能导致苯酚消费者与以降解产物为食的微生物比例不同。
