Department of Zoology, Microbial Biotechnology Laboratory, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, Pakistan.
PMAS-arid Agriculture University Rawalpindi, Pakistan.
Curr Pharm Biotechnol. 2024;25(10):1304-1320. doi: 10.2174/1389201024666230818124025.
The current study aimed to develop an economic plant-based therapeutic agent to improve the treatment strategies for diseases at the nano-scale because Cancer and Diabetes mellitus are major concerns in developing countries. Therefore, and antidiabetic and anti-cancerous activities of conjugated silver nanoparticles were assessed.
In the current study synthesis of silver nanoparticles using and characterization were done using a scanning electron microscope, UV-visible spectrophotometer, and FTIR analysis. The and anti-diabetic and anti-cancerous potential (200 mg/kg and 400 mg/kg) were carried out.
It was discovered that Balb/c mice did not show any major alterations during observation of acute oral toxicity when administered orally both TGaqu (1000 mg/kg) and TGAgNPs (1000 mg/kg), and results revealed that 1000 mg/kg is not lethal dose as did not find any abnormalities in epidermal and dermal layers when exposed to TGAgNPs. studies showed that TGAgNPs could not only inhibit alpha-glucosidase and protein kinases but were also potent against the brine shrimp. Though, a significant reduction in blood glucose levels and significant anti-cancerous effects was recorded when alloxan-treated and CCl-induced mice were treated with TGAgNPs and TGaqu.
Both in vivo and studies revealed that TGaqu and TGAgNPs are not toxic at 200 mg/kg, 400 mg/kg, and 1000 mg/kg doses and possess strong anti-diabetic and anti-cancerous effects due to the presence of phyto-constituents. Further, suggesting that green synthesized silver nanoparticles could be used in pharmaceutical industries to develop potent therapeutic agents.
本研究旨在开发一种基于经济植物的治疗剂，以改善纳米尺度疾病的治疗策略，因为癌症和糖尿病是发展中国家的主要关注点。因此，评估了共轭银纳米粒子的抗糖尿病和抗癌活性。
在本研究中，使用扫描电子显微镜、紫外可见分光光度计和傅里叶变换红外分析对银纳米粒子的合成和表征进行了研究。进行了 及 抗糖尿病和抗癌活性（200mg/kg 和 400mg/kg）研究。
发现Balb/c 小鼠在口服给予 TGaqu（1000mg/kg）和 TGAgNPs（1000mg/kg）时，在急性口服毒性观察中没有出现任何重大变化，结果表明 1000mg/kg 不是致死剂量，因为暴露于 TGAgNPs 时没有发现表皮和真皮层的异常。 研究表明，TGAgNPs 不仅可以抑制α-葡萄糖苷酶和蛋白激酶，而且对盐水虾也有很强的抑制作用。然而，当用 TGAgNPs 和 TGaqu 治疗链脲佐菌素和 CCl 诱导的糖尿病小鼠时，观察到血糖水平显著降低和显著的抗癌作用。
体内和 研究均表明，在 200mg/kg、400mg/kg 和 1000mg/kg 剂量下，TGaqu 和 TGAgNPs 没有毒性，并且由于存在植物成分，具有很强的抗糖尿病和抗癌作用。进一步表明，绿色合成的银纳米粒子可用于制药工业，以开发有效的治疗剂。
