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内皮细胞和雪旺细胞来源的外泌体的比较蛋白质组学分析。

Comparative proteomic analysis of exosomes derived from endothelial cells and Schwann cells.

机构信息

Department of Neurology, Henry Ford Health, Detroit, Michigan, United States of America.

Department of Physics, Oakland University, Rochester, Michigan, United States of America.

出版信息

PLoS One. 2023 Aug 18;18(8):e0290155. doi: 10.1371/journal.pone.0290155. eCollection 2023.

DOI:10.1371/journal.pone.0290155
PMID:37594969
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10437921/
Abstract

Exosomes derived from endothelial cells and Schwann cells have been employed as novel treatments of neurological diseases, including peripheral neuropathy. Exosomal cargo plays a critical role in mediating recipient cell function. In this study, we thus performed a comprehensive proteomic analysis of exosomes derived from healthy mouse dermal microvascular endothelial cells (EC-Exo) and healthy mouse Schwann cells (SC-Exo). We detected 1,817and 1,579 proteins in EC-Exo and SC-Exo, respectively. Among them, 1506 proteins were present in both EC-Exo and SC-Exo, while 311 and 73 proteins were detected only in EC-Exo and SC-Exo, respectively. Bioinformatic analysis revealed that EC-Exo enriched proteins were involved in neurovascular function, while SC-Exo enriched proteins were related to lipid metabolism. Western blot analysis of 14 enriched proteins revealed that EC-Exo contained proteins involved in mediating endothelial function such as delta-like 4 (DLL4) and endothelial NOS (NOS3), whereas SC-Exo had proteins involved in mediating glial function such as apolipoprotein A-I (APOA1) and phospholipid transfer protein (PLTP). Collectively, the present study identifies differences in the cargo protein profiles of EC-Exo and SC-Exo, thus providing new molecular insights into their biological functions for the treatment of peripheral neuropathy.

摘要

内皮细胞和雪旺细胞衍生的外泌体已被用作治疗神经疾病的新方法，包括周围神经病。外泌体货物在介导受体细胞功能方面起着关键作用。在这项研究中，我们对来自健康小鼠皮肤微血管内皮细胞（EC-Exo）和健康小鼠雪旺细胞（SC-Exo）的外泌体进行了全面的蛋白质组学分析。我们分别在 EC-Exo 和 SC-Exo 中检测到 1817 和 1579 种蛋白质。其中，1506 种蛋白质存在于 EC-Exo 和 SC-Exo 中，而 311 和 73 种蛋白质分别仅存在于 EC-Exo 和 SC-Exo 中。生物信息学分析显示，EC-Exo 富含的蛋白质参与神经血管功能，而 SC-Exo 富含的蛋白质与脂质代谢有关。对 14 种富集蛋白的 Western blot 分析显示，EC-Exo 含有参与调节内皮功能的蛋白，如 delta-like 4 (DLL4) 和内皮型一氧化氮合酶 (NOS3)，而 SC-Exo 含有参与调节神经胶质功能的蛋白，如载脂蛋白 A-I (APOA1) 和磷脂转移蛋白 (PLTP)。总之，本研究鉴定了 EC-Exo 和 SC-Exo 中货物蛋白谱的差异，从而为它们在治疗周围神经病中的生物学功能提供了新的分子见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/ac7872efeb8c/pone.0290155.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/64268a4e85c4/pone.0290155.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/f48b84903d26/pone.0290155.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/27039af4c683/pone.0290155.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/f24d2eb20ea2/pone.0290155.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/863f5f477336/pone.0290155.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/1f63028cc55d/pone.0290155.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/ac7872efeb8c/pone.0290155.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/64268a4e85c4/pone.0290155.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/f48b84903d26/pone.0290155.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/27039af4c683/pone.0290155.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/f24d2eb20ea2/pone.0290155.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/863f5f477336/pone.0290155.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/1f63028cc55d/pone.0290155.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7257/10437921/ac7872efeb8c/pone.0290155.g007.jpg

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Endothelial cells promote the proliferation and migration of Schwann cells.内皮细胞促进雪旺细胞的增殖和迁移。
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Voltage Dependent Anion Channel 3 (VDAC3) protects mitochondria from oxidative stress.电压依赖性阴离子通道3（VDAC3）可保护线粒体免受氧化应激。
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