Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.
Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.
PLoS One. 2023 Aug 18;18(8):e0289612. doi: 10.1371/journal.pone.0289612. eCollection 2023.
Native breeds in any country are a national capital, and their preservation is of great importance. Native Cattle of Guilan (NCG) is one of the few pure native breeds in Iran and the West Asia region. During the last decade, NCG population has decreased by more than 40%. This study aimed to identify significant single nucleotide polymorphisms (SNPs) and candidate genes associated with meat production traits in NCG using a genome-wide association study (GWAS). The blood and hair samples were collected from 72 NCG individuals and genotyped using the Illumina Bovine SNP50 chip. The results of the genomic scan showed that several SNPs were associated with abdominal depth, head width, hip width, and withers height in NCG. Several candidate genes were identified, including multiple epidermal growth factor-like domains 11 (MEGF11), Methionine Sulfoxide Reductase A (MSRA), chondroitin sulfate synthase 3 (CHSY3), Cyclin-Dependent Kinase 7 (CDK7), and Parkin (PRKN) genes, which are involved in muscle growth, meat tenderness, differentiation of fat cells, fat metabolism, and adipogenesis. These genes can contribute to meat quantity and quality in NCG. This study provided valuable insights into the genetics of NCG and the identification of effective genes associated with meat production traits. The results of this study could be used for the preservation and sustainable use of this breed of native cattle, as an important genetic resource in Iran.
任何国家的本土品种都是国家资本，保护它们非常重要。吉兰牛（NCG）是伊朗和西亚地区少数几个纯本土品种之一。在过去十年中，NCG 的数量减少了 40%以上。本研究旨在使用全基因组关联研究（GWAS）鉴定与 NCG 肉产量性状相关的显著单核苷酸多态性（SNP）和候选基因。从 72 头 NCG 个体中采集血液和毛发样本，并使用 Illumina Bovine SNP50 芯片进行基因分型。基因组扫描的结果表明，一些 SNP 与 NCG 的腹部深度、头部宽度、臀部宽度和肩高有关。鉴定出了几个候选基因，包括多个表皮生长因子样结构域 11（MEGF11）、甲硫氨酸亚砜还原酶 A（MSRA）、软骨素硫酸合酶 3（CHSY3）、细胞周期蛋白依赖性激酶 7（CDK7）和 Parkin（PRKN）基因，这些基因参与肌肉生长、肉质嫩度、脂肪细胞分化、脂肪代谢和脂肪生成。这些基因可以促进 NCG 的肉量和肉质。本研究为 NCG 的遗传学和与肉产量性状相关的有效基因的鉴定提供了有价值的见解。本研究的结果可用于保护和可持续利用这种本土牛品种，因为它是伊朗的一个重要遗传资源。
