Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, PR China.
Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Sichuan, 641100, PR China.
Chemosphere. 2023 Nov;340:139853. doi: 10.1016/j.chemosphere.2023.139853. Epub 2023 Aug 16.
Frequent detection of thiamethoxam in global surface waters has provoked great concern in environmental safety, as thiamethoxam exhibits high toxicity to aquatic arthropods. However, little systematic investigation has been conducted on the chronic toxicity of thiamethoxam to crustaceans. This study exposed Eriocheir sinensis to thiamethoxam (0, 0.5, 5 and 50 μg/L) in water for 28 days. No significant difference in mortality was observed among all groups. A high concentration of thiamethoxam (50 μg/L) impaired the righting ability of E. sinensis. Thiamethoxam significantly increased antioxidant enzyme activities (superoxide dismutase, total antioxidant capacity and glutathione peroxidase) and malondialdehyde levels. Simultaneously, detoxification enzyme activities (aminopyrine N-demethylase, erythromycin N-demethylase and glutathione-S-transferase) increased under chronic thiamethoxam stress. In addition, thiamethoxam caused immune and hepatopancreas damage. Moreover, thiamethoxam induced intestinal flora dysbiosis by altering the microbiome structure. The reduced complexity of the gut microbiota further illustrated that thiamethoxam could disrupt the stability of the microbiota ecological network. The transcriptomic results revealed that the number of downregulated DEGs increased in a dose-dependent manner, and most downregulated DEGs were enriched in energy metabolism-related pathways. These results indicate that thiamethoxam can adversely affect the crab behavior, biochemistry, intestinal microflora and transcriptomic responses.
频繁在全球地表水检测到噻虫嗪，这引起了人们对环境安全的极大关注，因为噻虫嗪对水生节肢动物具有很高的毒性。然而，目前对噻虫嗪对甲壳类动物的慢性毒性还没有进行系统的研究。本研究将中华绒螯蟹暴露于水中的噻虫嗪（0、0.5、5 和 50μg/L）中 28 天。所有组之间的死亡率均无显著差异。高浓度的噻虫嗪（50μg/L）损害了中华绒螯蟹的翻身能力。噻虫嗪显著增加了抗氧化酶活性（超氧化物歧化酶、总抗氧化能力和谷胱甘肽过氧化物酶）和丙二醛水平。同时，在慢性噻虫嗪胁迫下，解毒酶活性（氨基比林 N-去甲基酶、红霉素 N-去甲基酶和谷胱甘肽-S-转移酶）增加。此外，噻虫嗪还会导致免疫和肝胰腺损伤。此外，噻虫嗪通过改变微生物组结构引起肠道菌群失调。肠道微生物群落复杂性的降低进一步表明，噻虫嗪可以破坏微生物群落生态网络的稳定性。转录组结果表明，下调差异基因的数量呈剂量依赖性增加，大多数下调差异基因富集在与能量代谢相关的途径中。这些结果表明，噻虫嗪会对蟹的行为、生物化学、肠道微生物群和转录组反应产生不利影响。
