Ki Dae-Won, Kim Chae-Won, Choi Dae-Cheol, Oh Gun-Woo, Doan Thi-Phuong, Kim Ji-Yul, Oh Won-Keun, Lee In-Kyoung, Yun Bong-Sik
Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Gobong-ro 79, Iksan, 54596, South Korea.
Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Gobong-ro 79, Iksan, 54596, South Korea.
Phytochemistry. 2023 Oct;214:113828. doi: 10.1016/j.phytochem.2023.113828. Epub 2023 Aug 16.
Seven undescribed compounds, dentipellinones A‒D (1, 2, 5, and 6), dentipellinol (3), methoxyerinaceolactone B (4), and erinaceolactomer A (7), were isolated from the culture broth of Dentipellis fragilis. Chemical structures of these isolated compounds were determined by analyses of 1D and 2D-NMR and MS data in comparison with data reported in the literature. Absolute configurations of 1‒7 were also determined by Electronic Circular Dichroism calculations. The isolated compounds were evaluated for their anti-inflammatory effects on NO production and pro-inflammatory cytokines levels in LPS-stimulated RAW264.7 cells. Compounds 5 and 7 were evaluated for their anti-inflammatory effects on NO production and pro-inflammatory cytokine levels in LPS-stimulated RAW264.7 cells. They exhibited inhibitory effects on LPS-induced NO production in a dose-dependent manner, and significantly reduced the levels of inflammatory-related cytokines such as IL-1β and IL-6. TNF-α was not involved in the anti-inflammatory effects of these compounds. Finally, compounds 5 and 7 showed significant anti-inflammatory effects.
从脆弱齿菌的培养液中分离出了7种未描述的化合物，即齿菌素A-D（1、2、5和6）、齿菌醇（3）、甲氧基刺猬内酯B（4）和刺猬内酯异构体A（7）。通过对一维和二维核磁共振及质谱数据的分析，并与文献报道的数据进行比较，确定了这些分离出的化合物的化学结构。还通过电子圆二色性计算确定了1-7的绝对构型。评估了分离出的化合物对脂多糖刺激的RAW264.7细胞中一氧化氮生成和促炎细胞因子水平的抗炎作用。评估了化合物5和7对脂多糖刺激的RAW264.7细胞中一氧化氮生成和促炎细胞因子水平的抗炎作用。它们对脂多糖诱导的一氧化氮生成呈剂量依赖性抑制作用，并显著降低了白细胞介素-1β和白细胞介素-6等炎症相关细胞因子的水平。肿瘤坏死因子-α不参与这些化合物的抗炎作用。最后，化合物5和7显示出显著的抗炎作用。
