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经颅聚焦超声诱导无毛小鼠血脑屏障开放。

Transcranial focused ultrasound-induced blood‒brain barrier opening in mice without shaving hairs.

机构信息

Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO, 63130, USA.

Department of Neurosurgery, Washington University School of Medicine, Saint Louis, MO, 63110, USA.

出版信息

Sci Rep. 2023 Aug 19;13(1):13500. doi: 10.1038/s41598-023-40598-4.

Abstract

Acoustic coupling through hairs remains a challenge to performing transcranial-focused ultrasound procedures. Here, we demonstrated that this challenge could be addressed by using oil as the coupling medium, leveraging oil's high affinity to hairs due to their inherent hydrophobicity. We compared focused ultrasound-induced blood-brain barrier opening (FUS-BBBO) outcomes in mice under three coupling conditions: oil with hairs ("oil + hairs"), ultrasound gel with hair shaving ("ultrasound gel + no hair"), and ultrasound gel with hairs ("ultrasound gel + hairs"). The quality of the coupling was evaluated by [Formula: see text]-weighted magnetic resonance imaging (MRI) and passive cavitation detection (PCD). The outcome of FUS-BBBO was assessed by MRI contrast agent extravasation using in vivo [Formula: see text]-weighted contrast-enhanced MRI. It was also evaluated by ex vivo fluorescence imaging of the mouse brain after intravenous injection of a model drug, Evans blue. The results showed that "oil + hairs" consistently achieved high-quality acoustic coupling without trapping air bubbles. The FUS-BBBO outcome was not significantly different between the "oil + hairs" and the "ultrasound gel + no hair" groups. These two groups had significantly higher levels of BBB opening than the "ultrasound gel + hairs" group. This study demonstrated that oil could be a coupling medium for transcranial FUS procedures without shaving hairs.

摘要

通过毛发进行声耦合仍然是进行颅穿透聚焦超声手术的一个挑战。在这里,我们证明可以通过使用油作为耦合介质来解决这一挑战,这是因为油由于其固有的疏水性而对毛发具有很高的亲和力。我们在三种耦合条件下比较了在小鼠中进行的聚焦超声诱导的血脑屏障开放(FUS-BBBO)的结果:有毛发的油(“油+毛发”)、有毛发剃毛的超声凝胶(“超声凝胶+无毛发”)和有毛发的超声凝胶(“超声凝胶+毛发”)。使用[Formula: see text]-加权磁共振成像(MRI)和被动空化检测(PCD)评估耦合质量。通过使用体内[Formula: see text]-加权对比增强 MRI 评估 FUS-BBBO 的结果来评估 MRI 对比剂外渗。还通过静脉注射模型药物伊文思蓝后对小鼠脑进行离体荧光成像来评估 FUS-BBBO 的结果。结果表明,“油+毛发”可始终实现高质量的声耦合,而不会捕获气泡。“油+毛发”和“超声凝胶+无毛发”两组之间的 FUS-BBBO 结果没有显着差异。这两组的 BBB 开放程度明显高于“超声凝胶+毛发”组。这项研究表明,油可以作为无毛发剃除的颅穿透超声手术的耦合介质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac85/10439893/2f29549a8ea4/41598_2023_40598_Fig1_HTML.jpg

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