Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China.
Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China.
Food Chem. 2024 Jan 15;431:137117. doi: 10.1016/j.foodchem.2023.137117. Epub 2023 Aug 7.
Senescence is happening in every corner of the living organisms. β-galactosidase (β-gal) is one of the most important biomarkers during senescence in both plant and mammalian cells. Most β-gal fluorescent probes were focused on bio-imaging, only a few probes were developed for the detection of β-gal in fruit, and the probes that could detect β-gal in both fruits and living cells were even less. Here, two β-gal probes (TNap-βGal and TBNap-βGal) were synthesized, which can not only image the increase of β-gal during both fruits softening and cellular senescence, but also prove that bananas are not suitable for storage in refrigerator and the subsequent accumulation of β-gal still in lysosome of mammalian cells. In addition, TNap-βGal was successfully applied to two-photon imaging of endogenous β-gal in both hDPMSCs and tissues of human dental pulp for the first time.
衰老存在于生物体的每一个角落。β-半乳糖苷酶（β-gal）是植物和哺乳动物细胞衰老过程中最重要的生物标志物之一。大多数β-gal 荧光探针都集中在生物成像上，只有少数探针被开发用于检测水果中的β-gal，而能够检测水果和活细胞中β-gal 的探针则更少。在这里，合成了两种β-gal 探针（TNap-βGal 和 TBNap-βGal），它们不仅可以成像水果软化和细胞衰老过程中β-gal 的增加，还证明香蕉不适合存放在冰箱中，并且哺乳动物细胞溶酶体中β-gal 的积累仍在继续。此外，TNap-βGal 首次成功地应用于人牙髓间充质干细胞和人牙髓组织内源性β-gal 的双光子成像。
