Castilho Tiago Jacometo Coelho de, Almeida Gustavo Henrique Doná Rodrigues de, Mello Eneri Vieira de S L, Campos Antônio Carlos L
Postgraduate Program in Surgical Clinic, Health Sciences Sector, Federal University of Paraná, Curitiba, PR, Brazil.
Animal Histotechnical Laboratory, Department of Morphophysiological Sciences, State University of Maringá, Maringá, PR, Brazil.
Surg Open Sci. 2023 Jun 28;14:22-30. doi: 10.1016/j.sopen.2023.06.007. eCollection 2023 Aug.
The use of probiotics positively modifies the composition and function of the intestinal flora, decreasing inflammation, and these changes improve the quality of intestinal anastomosis. Therefore, the objective of this study was to evaluate the metagenomics of the microbial community after probiotic supplementation in rats subjected to intestinal anastomosis.
The probiotic chosen for this study was composed of the strains LPC37, HN0019, HN001 and NCFM. Both groups underwent two colostomies, one in the right colon and the second in the rectosigmoid colon, followed by anastomosis with eight interrupted stitches. The rats were killed on the fifth day of PO. Changes in the intestinal microbiota were evaluated by means of a metagenomic study that evaluated bacterial alpha and beta diversity indices.
Although there were no significant differences for any alpha diversity index, changes were observed for beta diversity indexes in the microbiota of rats. The group that received the probiotic preserved and even increased the abundance of beneficial bacterial genera and, at the same time, decreased the abundance of potentially pathogenic bacteria, promoting a favorable environment for anastomoses' healing.
The use of probiotics had a positive impact on the quality of the intestinal microbiota.
益生菌的使用可积极改变肠道菌群的组成和功能，减轻炎症，而这些变化可改善肠道吻合口的质量。因此，本研究的目的是评估在接受肠道吻合术的大鼠中补充益生菌后微生物群落的宏基因组学。
本研究选用的益生菌由LPC37、HN0019、HN001和NCFM菌株组成。两组均进行了两次结肠造口术，一次在右结肠，另一次在直肠乙状结肠，然后用八针间断缝合进行吻合。大鼠在术后第5天处死。通过评估细菌α和β多样性指数的宏基因组学研究来评估肠道微生物群的变化。
虽然任何α多样性指数均无显著差异，但在大鼠微生物群的β多样性指数上观察到了变化。接受益生菌的组保留甚至增加了有益细菌属的丰度，同时降低了潜在病原菌的丰度，为吻合口愈合创造了有利环境。
益生菌的使用对肠道微生物群的质量有积极影响。
