Zhao Minghan, Han Yuting, Yang Qi, Yue Qiulin, Zhang Song, Zhao Chen, Sun Xin, Xu Jing, Jiang Xingtao, Li Kunlun, Li Baojun, Zhao Lin, Su Le
State Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, P. R. China.
Shengshengxiangrong (Shandong) Biotechnology Co., Ltd., Jinan 250000, P. R. China.
ACS Omega. 2023 Aug 1;8(32):29336-29345. doi: 10.1021/acsomega.3c02758. eCollection 2023 Aug 15.
With the advancement of society, electronic cigarettes (e-cigarettes) have gained popularity among a growing number of individuals. While numerous toxicological studies have suggested that e-cigarettes are a safer alternative to traditional cigarettes, there is also a body of literature presenting contrasting findings. This study aimed to compare the effects of e-cigarettes and tobacco cigarettes (t-cigarettes) on RAW264.7 cells by using four e-cigarette aerosol extracts (ECA) and cigarette smoking extracts (CS) containing different nicotine concentrations. The results revealed that low concentration of nicotine in CS as well as in ECA with grape, watermelon, and cola flavors could promote cell viability. Conversely, high nicotine concentration in CS and ECA with four flavors decreased cell viability. Furthermore, our study demonstrated that CS significantly reduced the phagocytic capability of RAW264.7 cells and increased the levels of inflammatory cytokines (IL-6, TNF-α, and IL-1β) and reactive oxygen species (ROS) compared to ECA. Overall, our findings indicate all four e-cigarettes induced less cytotoxicity to RAW264.7 cells and might be safer than t-cigarettes.
随着社会的发展，电子烟在越来越多的人当中受到欢迎。虽然众多毒理学研究表明电子烟是比传统香烟更安全的替代品，但也有一批文献呈现出相反的研究结果。本研究旨在通过使用四种含有不同尼古丁浓度的电子烟气溶胶提取物（ECA）和香烟烟雾提取物（CS），比较电子烟和烟草香烟（T香烟）对RAW264.7细胞的影响。结果显示，CS以及葡萄味、西瓜味和可乐味ECA中低浓度的尼古丁可促进细胞活力。相反，CS和四种口味ECA中的高尼古丁浓度会降低细胞活力。此外，我们的研究表明，与ECA相比，CS显著降低了RAW264.7细胞的吞噬能力，并增加了炎性细胞因子（IL-6、TNF-α和IL-1β）和活性氧（ROS）的水平。总体而言，我们的研究结果表明，所有四种电子烟对RAW264.7细胞诱导的细胞毒性较小，可能比T香烟更安全。
