Milgate Andrew, Baxter Brad, Simpfendorfer Steven, Giblot-Ducray Daniele, Yang Nannan, Orchard Beverly, Ovenden Ben
NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW, Australia.
NSW Department of Primary Industries, Tamworth Agricultural Institute, Tamworth, NSW, Australia.
Front Plant Sci. 2023 Aug 2;14:1225283. doi: 10.3389/fpls.2023.1225283. eCollection 2023.
Fusarium crown rot (FCR) causes significant grain yield loss in winter cereals around the world. Breeding for resistance and/or tolerance to FCR has been slow with relatively limited success. In this study, multi-species experiments were used to demonstrate an improved method to quantify FCR infection levels at plant maturity using quantitative PCR (qPCR), as well as the genotype yield retention using residual regression deviation. Using qPCR to measure FCR infection allowed a higher degree of resolution between genotypes than traditional visual stem basal browning assessments. The results were consistent across three environments with different levels of disease expression. The improved measure of FCR infection along with genotype yield retention allows for partitioning of both tolerance and partial resistance. Together these methods offer new insights into FCR partial resistance and its relative importance to tolerance in bread wheat and barley. This new approach offers a more robust, unbiased way to select for both FCR traits within breeding programs. Genetic gain for tolerance and partial resistance against Fusarium crown rot (FCR) in winter cereals has been impeded by laborious and variable visual measures of infection severity. This paper presents results of an improved method to quantify FCR infection that are strongly correlated to yield loss and reveal previously unrecognised partial resistance in barley and wheat varieties.
镰刀菌冠腐病（FCR）在全球范围内导致冬季谷物的产量大幅损失。针对FCR抗性和/或耐受性的育种进展缓慢，成效相对有限。在本研究中，通过多物种实验展示了一种改进方法，即利用定量PCR（qPCR）在植株成熟时量化FCR感染水平，以及利用残差回归偏差量化基因型产量保持率。与传统的茎基部目测褐变评估相比，使用qPCR测量FCR感染能在基因型之间实现更高分辨率。在三种具有不同病害表现水平的环境中，结果都是一致的。对FCR感染的改进测量以及基因型产量保持率能够区分耐受性和部分抗性。这些方法共同为FCR部分抗性及其在面包小麦和大麦中对耐受性的相对重要性提供了新见解。这种新方法为在育种计划中选择FCR性状提供了一种更稳健、无偏差的方式。由于感染严重程度的目测测量繁琐且可变，冬季谷物对镰刀菌冠腐病（FCR）的耐受性和部分抗性的遗传增益受到了阻碍。本文介绍了一种改进的量化FCR感染的方法的结果，该方法与产量损失密切相关，并揭示了大麦和小麦品种中以前未被认识到的部分抗性。
