Wu Tianci, Guo Feilong, Xu Gangbiao, Yu Jinfeng, Zhang Li, Wei Xuening, Zhu Xiuliang, Zhang Zengyan
Institute of Crop Sciences, The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
The Laboratory of Forestry Genetics, Central South University of Forestry and Technology, Changsha 410004, China.
Biology (Basel). 2021 Nov 1;10(11):1122. doi: 10.3390/biology10111122.
The fungus can cause the destructive disease Fusarium crown rot (FCR) of wheat, an important staple crop. Functional roles of FCR resistance genes in wheat are largely unknown. In the current research, we characterized the antifungal activity and positive-regulatory function of the cysteine-rich repeat receptor-like kinase TaCRK-7A in the defense against in wheat. Antifungal assays showed that the purified TaCRK-7A protein inhibited the growth of . transcript abundance was elevated after attack and was positively related to FCR-resistance levels of wheat cultivars. Intriguingly, knocking down of transcript increased susceptibility of wheat to FCR and decreased transcript levels of defense-marker genes in wheat. Furthermore, the transcript abundances of and its modulated-defense genes were responsive to exogenous jasmonate treatment. Taken together, these results suggest that TaCRK-7A can directly inhibit growth and mediates FCR-resistance by promoting the expression of wheat defense genes in the jasmonate pathway. Thus, is a potential gene resource in FCR-resistant wheat breeding program.
这种真菌会引发小麦的毁灭性病害——镰刀菌冠腐病（FCR），小麦是一种重要的主粮作物。FCR抗性基因在小麦中的功能作用在很大程度上尚不清楚。在当前的研究中，我们对富含半胱氨酸重复序列的受体样激酶TaCRK - 7A在小麦防御中的抗真菌活性和正向调节功能进行了表征。抗真菌试验表明，纯化的TaCRK - 7A蛋白抑制了[具体真菌名称未给出]的生长。[具体基因名称未给出]的转录本丰度在[具体真菌名称未给出]侵染后升高，且与小麦品种的FCR抗性水平呈正相关。有趣的是，[具体基因名称未给出]转录本的敲低增加了小麦对FCR的易感性，并降低了小麦中防御标记基因的转录水平。此外，[具体基因名称未给出]及其调控的防御基因的转录本丰度对外源茉莉酸处理有响应。综上所述，这些结果表明TaCRK - 7A可以直接抑制[具体真菌名称未给出]的生长，并通过促进茉莉酸途径中小麦防御基因的表达来介导FCR抗性。因此，[具体基因名称未给出]是FCR抗性小麦育种计划中的一个潜在基因资源。
