Ocloo Remous, Newton-Foot Mae, Ziebuhr Wilma, Whitelaw Andrew Christopher
Division of Medical Microbiology and Immunology, Department of Pathology, Stellenbosch University, Stellenbosch, South Africa.
National Health Laboratory Service, Tygerberg Hospital, Cape Town, South Africa.
Front Microbiol. 2023 Aug 2;14:1239666. doi: 10.3389/fmicb.2023.1239666. eCollection 2023.
Staphylococci other than (SOSA) have emerged as significant pathogens in healthcare settings, particularly among patients with indwelling devices and immunocompromised individuals. and are the most common commensal SOSA species and are implicated in infections such as endocarditis and bacteremia. SOSA infections in neonates and children have been reported globally. Recent increases in antibiotic resistance and virulence among SOSA strains in clinical settings have highlighted the need to describe the reservoirs of SOSA to enable monitoring of these emerging pathogens.
Stool samples were collected from 150 healthy children from Cape Town communities between 2017 and 2020. Staphylococci were isolated, identified using mass-spectrometry, and antimicrobial susceptibility testing and Illumina whole genome sequencing were performed.
Among the participants, 50 (33.3%) were colonized by SOSA, with ( = 38; 25.3%) being the most common, followed by ( = 5; 3.3%) and ( = 5; 3.3%). Out of the 77 initially isolated strains, 23 were identified as through whole genome sequencing. All isolates ( = 49) were methicillin resistant, with 65.3% ( = 32) harbouring . In , SCC type VIII(4A) was detected in 42.0% of ST9 isolates while non- methicillin resistant isolates were mostly ST49 (41.1%). Additionally, 16 (50.0%) strains contained non-typeable SCC elements.
High rates of methicillin resistance were identified among colonizing SOSA in Cape Town, increasing the risk of transmission to clinical settings. This study also identified a new species, , for the first time in Africa.
除金黄色葡萄球菌外的葡萄球菌（SOSA）已成为医疗机构中的重要病原体，尤其是在留置装置患者和免疫功能低下个体中。腐生葡萄球菌和表皮葡萄球菌是最常见的共生SOSA菌种，与心内膜炎和菌血症等感染有关。全球范围内均有新生儿和儿童SOSA感染的报道。临床环境中SOSA菌株抗生素耐药性和毒力的近期增加凸显了描述SOSA储存库以监测这些新兴病原体的必要性。
2017年至2020年期间，从开普敦社区的150名健康儿童中收集粪便样本。分离葡萄球菌，采用质谱法进行鉴定，并进行抗菌药物敏感性测试和Illumina全基因组测序。
在参与者中，50人（33.3%）被SOSA定植，其中腐生葡萄球菌（n = 38；25.3%）最为常见，其次是表皮葡萄球菌（n = 5；3.3%）和模仿葡萄球菌（n = 5；3.3%）。在最初分离的77株腐生葡萄球菌菌株中，通过全基因组测序鉴定出23株为模仿葡萄球菌。所有腐生葡萄球菌分离株（n = 49）均对甲氧西林耐药，其中65.3%（n = 32）携带mecA。在模仿葡萄球菌中，42.0%的ST9分离株检测到VIII型（4A）葡萄球菌染色体盒（SCC），而非甲氧西林耐药的模仿葡萄球菌分离株大多为ST49（41.1%）。此外，16株（50.0%）模仿葡萄球菌菌株含有不可分型的SCC元件。
在开普敦定植的SOSA中发现了较高的甲氧西林耐药率，增加了传播至临床环境的风险。本研究还首次在非洲鉴定出一个新菌种，即模仿葡萄球菌。
