García-Béjar Beatriz, García de Blas Martín Izan, Arévalo-Villena María, Briones Pérez Ana
Department of Analytical Chemistry and Food Technology, University of Castilla-La Mancha, Camilo José Cela Avenue, 13071 Ciudad Real, Spain.
Regional Institute of Applied Scientific Investigation (IRICA), University of Castilla-La Mancha, Camilo José Cela Avenue, 13071 Ciudad Real, Spain.
Animals (Basel). 2021 Nov 9;11(11):3197. doi: 10.3390/ani11113197.
The prevalence of was analysed in poultry products from different Spanish retailers and determined its antibiotic resistance capability by phenotypic (ampicillin, amoxicillin, chloramphenicol, gentamicin, imipenem, cefotaxime, tetracycline, ciprofloxacin, trimethoprim, and colistin) and genotypic assays. A total of 30 samples (hindquarters or livers) were collected from supermarkets and butchers. Enterobacteriaceae counts ranged between 3.2 and 6.5 log colony-forming units (CFU)/g, and the highest values were found in livers and in samples from supermarkets. was detected in 83% of the samples tested, and the highest prevalence was observed in livers (100%) and supermarkets (91%). Regarding the antibiotic sensitivity test, 100% of the showed resistance to at least one antibiotic. The highest resistance rates were detected for colistin (87%) and gentamicin (79%), while only two antibiotics (chloramphenicol and cefotaxime) showed a resistance lower than 10%. Furthermore, the resistance genes of tetracycline and beta-lactams were analysed by multiplex PCR, revealing that () and were the majority genes, respectively.
对来自不同西班牙零售商的家禽产品中的[未提及的内容]流行情况进行了分析，并通过表型（氨苄青霉素、阿莫西林、氯霉素、庆大霉素、亚胺培南、头孢噻肟、四环素、环丙沙星、甲氧苄啶和黏菌素）和基因型检测确定其抗生素耐药能力。总共从超市和肉店收集了30个样本（后腿或肝脏）。肠杆菌科细菌计数范围为3.2至6.5 log菌落形成单位（CFU）/克，在肝脏和超市样本中发现了最高值。在83%的测试样本中检测到了[未提及的内容]，在肝脏（100%）和超市（91%）中观察到了最高流行率。关于抗生素敏感性测试，1[未提及的内容]00%的[未提及的内容]对至少一种抗生素表现出耐药性。黏菌素（87%）和庆大霉素（79%）的耐药率最高，而只有两种抗生素（氯霉素和头孢噻肟）的耐药率低于10%。此外，通过多重PCR分析了四环素和β-内酰胺类的耐药基因，结果显示（[未提及的内容]）和[未提及的内容]分别是主要基因。
